Evidence map›Paper›PMID 35065666›Full record

ArticleSkeletal muscle2022

Novel γ-sarcoglycan interactors in murine muscle membranes.

Tara C Smith, Georgios Vasilakos, Scott A Shaffer, Jason M Puglise, Chih-Hsuan Chou, Elisabeth R Barton, Elizabeth J Luna

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Skeletal muscle, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Tara C SmithDepartment of Radiology, Division of Cell Biology & Imaging, University of Massachusetts Medical School, Worcester, MA, USA.
Georgios VasilakosApplied Physiology & Kinesiology, College of Health & Human Performance, University of Florida, Gainesville, FL, USA.
Scott A ShafferBiochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA.
Jason M PugliseApplied Physiology & Kinesiology, College of Health & Human Performance, University of Florida, Gainesville, FL, USA.
Chih-Hsuan ChouApplied Physiology & Kinesiology, College of Health & Human Performance, University of Florida, Gainesville, FL, USA.
Elisabeth R BartonApplied Physiology & Kinesiology, College of Health & Human Performance, University of Florida, Gainesville, FL, USA. erbarton@ufl.edu.
Elizabeth J LunaDepartment of Radiology, Division of Cell Biology & Imaging, University of Massachusetts Medical School, Worcester, MA, USA. Elizabeth.Luna@umassmed.edu.ORCID 0000-0002-0779-3379
University of Florida · USUniversity of Massachusetts Chan Medical School · US

Funding

The Sarcoglycan Complex in Skeletal Muscle MechanotransductionR01AR069660 · NIAMS · UNIVERSITY OF FLORIDA · PI BARTON, ELISABETH R · 2016 to 2020
$1.8M
NIAMS NIH HHS R01 AR069660NIH HHS R01-AR069660
6 · The paper itself

Abstract

backgroundThe sarcoglycan complex (SC) is part of a network that links the striated muscle cytoskeleton to the basal lamina across the sarcolemma. The SC coordinates changes in phosphorylation and Ca

methodsTo gain insight into how the SC mediates mechano-signaling in muscle, we utilized LC-MS/MS proteomics of SC-associated proteins in immunoprecipitates from enriched sarcolemmal fractions. Criteria for inclusion were co-immunoprecipitation with anti-Sgcg from C57BL/6 control muscle and under-representation in parallel experiments with Sgcg-null muscle and with non-specific IgG. Validation of interaction was performed in co-expression experiments in human RH30 rhabdomyosarcoma cells.

resultsWe identified 19 candidates as direct or indirect interactors for Sgcg, including the other 3 SC proteins. Novel potential interactors included protein-phosphatase-1-catalytic-subunit-beta (Ppp1cb, PP1b) and Na

conclusionsResults support that NKCC1 is a new SC-associated signaling protein. Moreover, the identities of other candidate SC interactors suggest ways by which the SC and NKCC1, along with other Sgcg interactors such as the membrane-cytoskeleton linker archvillin, may regulate kinase- and Ca

Indexed as

RhabdomyosarcomaSarcoglycansAnimalsChromatography, LiquidHumansMiceMuscle, SkeletalSolute Carrier Family 12, Member 2Tandem Mass SpectrometrySarcoglycansSLC12A2 protein, humanSolute Carrier Family 12, Member 2ArchvillinLimb girdle muscular dystrophyNKCC1PP1β/δSarcoglycansSarcolemmaSkeletal muscleSvil

Identifiers

PMID35065666
PMCPMC8783446
OpenAlexW4206897722

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.