Evidence map›Paper›PMID 35064858›Full record

ArticleJournal of fluorescence2022

Low-cost Imaging of Fluorescent DNA in Agarose Gel Electrophoresis using Raspberry Pi cameras.

Hassan Ali Abid, Jian Wern Ong, Eric Shen Lin, Zhixiong Song, Oi Wah Liew, Tuck Wah Ng

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of fluorescence, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Cordycepin EnhancesIn vivo (Athens, Greece)
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Hassan Ali AbidLaboratory for Optics, Department of Mechanical & Aerospace Engineering, Monash University, & Mechanics, AcousticsClayton, VIC, 3800, Australia.
Jian Wern OngLaboratory for Optics, Department of Mechanical & Aerospace Engineering, Monash University, & Mechanics, AcousticsClayton, VIC, 3800, Australia.
Eric Shen LinLaboratory for Optics, Department of Mechanical & Aerospace Engineering, Monash University, & Mechanics, AcousticsClayton, VIC, 3800, Australia.
Zhixiong SongLaboratory for Optics, Department of Mechanical & Aerospace Engineering, Monash University, & Mechanics, AcousticsClayton, VIC, 3800, Australia.
Oi Wah LiewCentre for Translational Medicine, Cardiovascular Research Institute, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, 14 Medical Drive, 117599, Singapore.
Tuck Wah NgLaboratory for Optics, Department of Mechanical & Aerospace Engineering, Monash University, & Mechanics, AcousticsClayton, VIC, 3800, Australia. engngtw@gmail.com.ORCID http://orcid.org/0000-0001-5592-1390

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Low-cost analytical solutions built around microcomputers like the Raspberry Pi help to facilitate laboratory investigations in resource limited venues. Here, three camera modules (V1.3 with and without filter, as well as NoIR) that work with this microcomputer were assessed for their suitability in imaging fluorescent DNA following agarose gel electrophoresis. Evaluation of their utility was based on signal-to-noise (SNR) and noise variance metrics that were developed. Experiments conducted with samples were subjected to Polymerase Chain Reaction (PCR), and the amplified products were separated using gel electrophoresis and stained with Midori green. Image analysis revealed the NoIR camera performed the best with SNR and noise variance values of 21.7 and 0.222 respectively. In experiments conducted using UV LED lighting to simulate ethidium bromide (EtBr) excitation, the NoIR and V1.3 with filter removed cameras showed comparable SNR values.

Indexed as

Fluorescent DyesMicrocomputersDNAElectrophoresis, Agar GelPolymerase Chain ReactionStaining and LabelingDNAFluorescent DyesAgaroseDeoxyribonucleic acidGel electrophoresisImagingPolymerase Chain Reaction

Identifiers

PMID35064858
PMCPMC8888377

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.