Evidence map›Paper›PMID 35064357›Full record

ArticleApplied microbiology and biotechnology2022

Characterization and heterologous expression of a novel Co

Puying Zhao, Meng Zhang, Xiaofu Wan, Peiling Geng, Hairong Xiong, Xiaomin Hu

Abstract read
PubMed Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Puying ZhaoCollege of Life Science, South-Central University for Nationalities, Wuhan, 430074, China.
Meng ZhangCollege of Life Science, South-Central University for Nationalities, Wuhan, 430074, China.
Xiaofu WanWuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
Peiling GengCollege of Life Science, South-Central University for Nationalities, Wuhan, 430074, China.
Hairong XiongCollege of Life Science, South-Central University for Nationalities, Wuhan, 430074, China. xionghr@mail.scuec.edu.cn.
Xiaomin HuCollege of Life Science, South-Central University for Nationalities, Wuhan, 430074, China. xiaomin.hu@mail.scuec.edu.cn.ORCID http://orcid.org/0000-0001-7361-5530
South Central Minzu University · CNChinese Academy of Sciences · CN

Funding

Fundamental Research Funds for Central Universities of South-Central University for Nationalities CZZ20001Hubei Natural Science Foundation 2021CFB201Hubei Province Innovation Major Program 2018ABA093National Natural Science Foundation of China (NFSC) 32170008
6 · The paper itself

Abstract

This study aims to explore the potential aminopeptidases of Lysinibacillus sphaericus based on the unique metabolic characteristics of this species which cannot metabolize carbohydrates and may have a strong ability to metabolize amino acids. Fifteen peptidase-encoding genes predicted in L. sphaericus C3-41 have been heterologously expressed in Escherichia coli BL21, and of these genes, only Amp0279 shows a high ability to hydrolyze L-leucine-4-nitroanilide (Leu-pNA). Phylogenetic analysis, 3D-structure modeling, and enzyme assays indicated that Amp0279 should be a novel Co

Indexed as

BacillaceaeBacillusLeucyl AminopeptidasePhylogenyLeucyl AminopeptidaseBacillus subtilisCorynebacterium glutamicumLeucyl aminopeptidaseLysinibacillus sphaericusSecretory expression

Identifiers

PMID35064357
OpenAlexW4206976087

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.