ArticleDevelopmental cell2022
An ex vivo system to study cellular dynamics underlying mouse peri-implantation development.
Article in Developmental cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- From Static to Dynamic: Fluorescence Imaging Technology Advances Precise Embryo Evaluation.Small methods · 2026Review
- Multicellular simulations with shape and volume constraints using optimal transport.Science advances · 2026Article
- Boundary geometry controls a topological defect transition that determines lumen nucleation in embryonic development.Nature materials · 2026Article
- A three-dimensional spatial transcriptome atlas reconstructs early organogenesis in primate Carnegie stages 9 and 10 embryos.Nature cell biology · 2026Article
- Mechanical cues regulateMaterials today. Bio · 2026Article
- Persistent homology-based segmentation tool for membrane images.Cell reports methods · 2026Article
- 3D biomimetic niche modulates embryo development in vitro.Nature communications · 2026Article
- Modelling mammalian implantationAnimal reproduction · 2026Review
- Boundary-guided cell alignment drives mouse epiblast maturation.Nature physics · 2026Article
- Review
- Traction force and mechanosensitivity mediate species-specific implantation patterns in human and mouse embryos.Science advances · 2025Article
- Maternal intestinal L. vaginalis facilitates embryo implantation and survival through enhancing uterine receptivity in sows.Microbiome · 2025Article
- Eomesodermin in conjunction with the BAF complex promotes expansion and invasion of the trophectoderm lineage.Nature communications · 2025Article
- Generation of transient totipotent blastomere-like stem cells by short-term high-dose Pladienolide B treatment.Science China. Life sciences · 2025Article
- Origin, fate and function of extraembryonic tissues during mammalian development.Nature reviews. Molecular cell biology · 2025Review
- Developing a Predictive Model for Metastatic Potential in Pancreatic Neuroendocrine Tumor.The Journal of clinical endocrinology and metabolism · 2024Article
- Novel imaging and biophysical approaches to study tissue hydraulics in mammalian folliculogenesis.Biophysical reviews · 2024Review
- The shapes of elongating gastruloids are consistent with convergent extension driven by a combination of active cell crawling and differential adhesion.PLoS computational biology · 2024Article
- A comprehensive review: synergizing stem cell and embryonic development knowledge in mouse and human integrated stem cell-based embryo models.Frontiers in cell and developmental biology · 2024Review
- The in vitro culture of mammalian embryos.Nature methods · 2023Article
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12 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Upon implantation, mammalian embryos undergo major morphogenesis and key developmental processes such as body axis specification and gastrulation. However, limited accessibility obscures the study of these crucial processes. Here, we develop an ex vivo Matrigel-collagen-based culture to recapitulate mouse development from E4.5 to E6.0. Our system not only recapitulates embryonic growth, axis initiation, and overall 3D architecture in 49% of the cases, but its compatibility with light-sheet microscopy also enables the study of cellular dynamics through automatic cell segmentation. We find that, upon implantation, release of the increasing tension in the polar trophectoderm is necessary for its constriction and invagination. The resulting extra-embryonic ectoderm plays a key role in growth, morphogenesis, and patterning of the neighboring epiblast, which subsequently gives rise to all embryonic tissues. This 3D ex vivo system thus offers unprecedented access to peri-implantation development for in toto monitoring, measurement, and spatiotemporally controlled perturbation, revealing a mechano-chemical interplay between extra-embryonic and embryonic tissues.
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