Evidence map›Paper›PMID 35063073›Full record

ArticleCell2022

Vision-dependent specification of cell types and function in the developing cortex.

Sarah Cheng, Salwan Butrus, Liming Tan, Runzhe Xu, Srikant Sagireddy, Joshua T Trachtenberg, Karthik Shekhar, S Lawrence Zipursky

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 97 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
97citing papers in PubMed, 1 pooled it
10.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

97 citing papers in PubMed, 1 synthesis or guideline pooled it, 127 citations in OpenAlex.

  1. Pooled it
  2. Excitatory and inhibitory networks diverge following early blindness.Cerebral cortex (New York, N.Y. : 1991) · 2026
    Article
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  4. Review
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  13. Binocular Circuitry as a Model for Understanding Experience-Dependent Circuit Development across the Mammalian Cortex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026
    Review
  14. Article
  15. Article
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37 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Sarah ChengDepartment of Biological Chemistry, Howard Hughes Medical Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Ophthalmology, Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Salwan ButrusDepartment of Chemical and Biomolecular Engineering, Helen Wills Neuroscience Institute, California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, Berkeley, CA 94720, USA.
Liming TanDepartment of Biological Chemistry, Howard Hughes Medical Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Runzhe XuDepartment of Biological Chemistry, Howard Hughes Medical Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Srikant SagireddyDepartment of Chemical and Biomolecular Engineering, Helen Wills Neuroscience Institute, California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, Berkeley, CA 94720, USA.
Joshua T TrachtenbergDepartment of Neurobiology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
Karthik ShekharDepartment of Chemical and Biomolecular Engineering, Helen Wills Neuroscience Institute, California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, Berkeley, CA 94720, USA; Faculty Scientist, Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address: kshekhar@berkeley.edu.
S Lawrence ZipurskyDepartment of Biological Chemistry, Howard Hughes Medical Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: lzipursky@mednet.ucla.edu.
Howard Hughes Medical Institute · USQB3 · USUniversity of California, Los Angeles · US

Funding

Origins of plasticity in the establishment of binocular visionR01EY023871 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Joshua Trachtenberg · 2013 to 2026
$5.4M
Uncovering the molecular identity of retinal cell types, and their responses to nerve injury using single-cell transcriptomicsR00EY028625 · NEI · UNIVERSITY OF CALIFORNIA BERKELEY · PI SHEKHAR, KARTHIK · 2020 to 2022
$740k
NEI NIH HHS R00 EY028625NEI NIH HHS R01 EY023871
6 · The paper itself

Abstract

The role of postnatal experience in sculpting cortical circuitry, while long appreciated, is poorly understood at the level of cell types. We explore this in the mouse primary visual cortex (V1) using single-nucleus RNA sequencing, visual deprivation, genetics, and functional imaging. We find that vision selectively drives the specification of glutamatergic cell types in upper layers (L) (L2/3/4), while deeper-layer glutamatergic, GABAergic, and non-neuronal cell types are established prior to eye opening. L2/3 cell types form an experience-dependent spatial continuum defined by the graded expression of ∼200 genes, including regulators of cell adhesion and synapse formation. One of these genes, Igsf9b, a vision-dependent gene encoding an inhibitory synaptic cell adhesion molecule, is required for the normal development of binocular responses in L2/3. In summary, vision preferentially regulates the development of upper-layer glutamatergic cell types through the regulation of cell-type-specific gene expression programs.

Indexed as

Vision, OcularAnimalsAnimals, NewbornBiomarkersgamma-Aminobutyric AcidGene Expression ProfilingGene Expression Regulation, DevelopmentalGlutamic AcidMaleMembrane ProteinsMiceMice, Inbred C57BLNerve Tissue ProteinsNeuronsRNA-SeqTranscriptomeBiomarkersgamma-Aminobutyric AcidGlutamic AcidIgSF9b protein, mouseMembrane ProteinsNerve Tissue Proteinsbinocular visioncell typescritical periodinhibitory synapseslayer 2/3single-nucleus RNA-seqvisual cortex

Identifiers

PMID35063073
PMCPMC8813006
OpenAlexW4226308686

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.