Evidence map›Paper›PMID 35063020›Full record

ArticleCell & bioscience2022

Transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty.

Shasha Zhou, Shaolian Zang, Yanping Hu, Yifen Shen, Hua Li, Wenlian Chen, Pin Li, Yihang Shen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell & bioscience, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
4.6field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 23 citations in OpenAlex.

  1. Review
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  4. Article
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  6. Article
  7. Review
  8. Disorders of puberty and neurodevelopment: A shared etiology?Annals of the New York Academy of Sciences · 2024
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Shasha Zhou *Central Laboratory, Suzhou Ninth People's Hospital, 2666, Ludang Road, Suzhou, 215200, Jiangsu, China.
Shaolian Zang *Department of Endocrinology, Shanghai Children's Hospital, Shanghai Jiao Tong University, 355 Luding Road, Shanghai, 200062, China.
Yanping Hu *Department of Molecular Pathology, The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, 450008, Henan, China.
Yifen Shen *Central Laboratory, Suzhou Ninth People's Hospital, 2666, Ludang Road, Suzhou, 215200, Jiangsu, China.
Hua LiBio-ID Center, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.
Wenlian ChenCancer Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China.
Pin LiDepartment of Endocrinology, Shanghai Children's Hospital, Shanghai Jiao Tong University, 355 Luding Road, Shanghai, 200062, China. lipinpub888@163.com.
Yihang ShenCentral Laboratory, Suzhou Ninth People's Hospital, 2666, Ludang Road, Suzhou, 215200, Jiangsu, China. devbrother@sjtu.edu.cn.ORCID http://orcid.org/0000-0002-2923-1641
Shanghai Jiao Tong University · CNSoochow University · CNShanghai University of Traditional Chinese Medicine · CNZhengzhou University · CN

Funding

Cultivation Plan for Excellent Young Talents of Shanghai Children's Hospital 2017YYQ05Hospital Level Project of Shanghai Children's Hospital 2018YQN002Key Technology Research and Development Program of Shandong 202102310443National Natural Science Foundation of China 81871131sponsored by the Interdisciplinary Program of Shanghai Jiao Tong University YG2021ZD25
6 · The paper itself

Abstract

backgroundA variety of neurons in hypothalamus undergo a complicated regulation on transcription activity of multiple genes for hypothalamic-pituitary-gonadal axis activation during pubertal development. Identification of puberty-associated cell composition and characterization of the unique transcriptional signatures across different cells are beneficial to isolation of specific neurons and advanced understanding of their functions.

methodsThe hypothalamus of female Sprague-Dawley rats in postnatal day-25, 35 and 45 were used to define the dynamic spatial atlas of gene expression in the arcuate nucleus (ARC) by 10× Genomics Visium platform. A surface protein expressed selectively by kisspeptin neurons was used to sort neurons by flow cytometric assay in vitro. The transcriptome of the isolated cells was examined using Smart sequencing.

resultsFour subclusters of neurons with similar gene expression signatures in ARC were identified. Only one subcluster showed the robust expression of Kiss1, which could be isolated by a unique membrane surface biomarker Solute carrier family 18 member A3 (SLC18A3). Moreover, genes in different subclusters presenting three expression modules distinctly functioned in each pubertal stage. Different types of cells representing distinct functions on glial or neuron differentiation, hormone secretion as well as estradiol response precisely affect and coordinate with each other, resulting in a complicated regulatory network for hypothalamic-pituitary-gonadal axis initiation and modulation.

conclusionOur data revealed a comprehensive transcriptomic overview of ARC within different pubertal stages, which could serve as a valuable resource for the study of puberty and sexual development disorders.

Indexed as

ARCHypothalamusKiss1PubertySlc18a3Spatial transcriptome

Identifiers

PMID35063020
PMCPMC8781439
OpenAlexW4205599705

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.