ArticleEpigenetics & chromatin2022
Effects of paternal exposure to cigarette smoke on sperm DNA methylation and long-term metabolic syndrome in offspring.
Article in Epigenetics & chromatin, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 59 citations in OpenAlex.
- Prenatal Smoking Exposures and Epigenome-Wide Methylation in Newborn Blood.Environmental health perspectives · 2026Pooled it
- Modifiable key factors and semen quality in men undergoing preconception evaluation: a cross-sectional study.Asian journal of andrology · 2026Article
- Review
- Fine particulate matter and tobacco product exposure exacerbates metabolic syndrome-related colon cancer via regulating oxidative stress and tumor-associated macrophage interactions.Cancer & metabolism · 2026Article
- Exposure of male mice to nicotine leads to metabolic dysfunction in their male and female offspring.Journal of the Endocrine Society · 2026Article
- Does a Father's Social Environment Influence Their Sons' Sperm Sex Ratio? Potential for the Epigenetic Transmission of a Sex-Allocating Mechanism.Ecology and evolution · 2025Article
- Impact of physical activity on semen quality: a review of current evidence.Asian journal of andrology · 2025Review
- Mechanisms and reversibility of nicotine-induced spermatogenesis impairment and DNA methylation changes.Communications biology · 2025Article
- Review
- Male aging in germ cells: What are we inheriting?Genetics and molecular biology · 2025Article
- The paternal role in pregnancy loss.Andrology · 2025Review
- Identification of Novel Proteins Mediating Causal Association Between Smoking and Essential Hypertension: A Mendelian Randomization Study.Journal of the American Heart Association · 2024Article
- Early life events may be the first steps on the multistep path to amyotrophic lateral sclerosis.Scientific reports · 2024Article
- Multifaceted paternal exposures before conception and their epigenetic impact on offspring.Journal of assisted reproduction and genetics · 2024Review
- Methylation alterations of imprinted genes in different placental diseases.Clinical epigenetics · 2024Article
- Influence of substance use on male reproductive health and offspring outcomes.Nature reviews. Urology · 2024Review
- Intergenerational association of DNA methylation between parents and offspring.Scientific reports · 2024Article
- Behind the Genetics: The Role of Epigenetics in Infertility-Related Testicular Dysfunction.Life (Basel, Switzerland) · 2024Review
- Pregnancy induced hypertension and umbilical cord blood DNA methylation in newborns: an epigenome-wide DNA methylation study.BMC pregnancy and childbirth · 2024Article
- Inter- and trans-generational impacts of environmental exposures on the germline resolved at the single-cell level.Current opinion in toxicology · 2024Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlthough paternal exposure to cigarette smoke may contribute to obesity and metabolic syndrome in offspring, the underlying mechanisms remain uncertain.
methodsIn the present study, we analyzed the sperm DNA-methylation profiles in tobacco-smoking normozoospermic (SN) men, non-tobacco-smoking normozoospermic (N) men, and non-smoking oligoasthenozoospermic (OA) men. Using a mouse model, we also analyzed global methylation and differentially methylated regions (DMRs) of the DLK1 gene in paternal spermatozoa and the livers of progeny. In addition, we quantified DLK1 expression, executed an intra-peritoneal glucose tolerance test (IPGTT), measured serum metabolites, and analyzed liver lipid accumulation in the F1 offspring.
resultsGlobal sperm DNA-methylation levels were significantly elevated (p < 0.05) in the SN group, and the methylation patterns were different among N, SN, and OA groups. Importantly, the methylation level of the DLK1 locus (cg11193865) was significantly elevated in the SN group compared to both N and OA groups (p < 0.001). In the mouse model, the group exposed to cigarette smoke extract (CSE) exhibited a significantly higher global methylation DNA level in spermatozoa (p < 0.001) and on the DMR sites of Dlk1 in 10-week-old male offspring (p < 0.05), with a significant increase in Dlk1 expression in their livers (p < 0.001). In addition, IPGTT and LDL levels were significantly altered (p < 0.001), with elevated liver fat accumulation (p < 0.05) in F1 offspring.
conclusionPaternal exposure to cigarette smoke led to increased global methylation of sperm DNA and alterations to the DMR of the DLK1 gene in the F1 generation, which may be inherited parentally and may perturb long-term metabolic function.
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