Evidence map›Paper›PMID 35062691›Full record

ArticleVaccines2021

mDrop-Seq: Massively Parallel Single-Cell RNA-Seq of

Ryan Dohn, Bingqing Xie, Rebecca Back, Alan Selewa, Heather Eckart, Reeta Prusty Rao, Anindita Basu

Open access · goldAbstract read
In one paragraph

Article in Vaccines, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Advances in Single-Cell Sequencing for Infectious Diseases: Progress and Perspectives.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Review
  6. Review
  7. Review
  8. Article
  9. Article
  10. eLife · 2023
    Article
  11. Review
  12. Article
  13. Fungal biotechnology: From yesterday to tomorrow.Frontiers in fungal biology · 2023
    Review
  14. Article
  15. Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Ryan DohnSection of Genetic Medicine, Department of Medicine, University of Chicago, Chicago, IL 60637, USA.
Bingqing XieSection of Genetic Medicine, Department of Medicine, University of Chicago, Chicago, IL 60637, USA.ORCID 0000-0001-9246-9321
Rebecca BackSection of Genetic Medicine, Department of Medicine, University of Chicago, Chicago, IL 60637, USA.ORCID 0000-0002-6649-2672
Alan SelewaSection of Genetic Medicine, Department of Medicine, University of Chicago, Chicago, IL 60637, USA.
Heather EckartSection of Genetic Medicine, Department of Medicine, University of Chicago, Chicago, IL 60637, USA.ORCID 0000-0002-9332-3073
Reeta Prusty RaoDepartment of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA 01609, USA.
Anindita BasuSection of Genetic Medicine, Department of Medicine, University of Chicago, Chicago, IL 60637, USA.
University of Chicago · USWorcester Polytechnic Institute · US

Funding

Profiling Transcriptional Heterogeneity in Microbial Cells at Single Cell resolution and High-throughput using Droplet MicrofluidicsDP2AI158157 · NIAID · UNIVERSITY OF CHICAGO · PI BASU, ANINDITA · 2020 to 2020
$2.4M
Novel microfluidic platform to profile host-pathogen interaction under controlled infection and single cell resolutionR21AI144417 · NIAID · UNIVERSITY OF CHICAGO · PI BASU, ANINDITA · 2019 to 2020
$416k
National Institute of Allergy and Infectious Diseases AI144417-02National Institute of Allergy and Infectious Diseases AI158157-01NIAID NIH HHS DP2 AI158157United States-Israel Binational Science Foundation 2017357
6 · The paper itself

Abstract

Advances in high-throughput single-cell RNA sequencing (scRNA-seq) have been limited by technical challenges such as tough cell walls and low RNA quantity that prevent transcriptomic profiling of microbial species at throughput. We present microbial Drop-seq or mDrop-seq, a high-throughput scRNA-seq technique that is demonstrated on two yeast species,

Indexed as

Candida albicanscell-cyclefluconazolehigh-throughputmDrop-seqSaccharomyces cerevisiaescRNA-seqstress response

Identifiers

PMID35062691
PMCPMC8779198
OpenAlexW4225881775

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.