Evidence map›Paper›PMID 35062047›Full record

ArticleChembiochem : a European journal of chemical biology2022

Optimized Hemolysin Type 1 Secretion System in Escherichia coli by Directed Evolution of the Hly Enhancer Fragment and Including a Terminator Region.

Zohreh Pourhassan N, Haiyang Cui, Sakshi Khosa, Mehdi D Davari, Karl-Erich Jaeger, Sander H J Smits, Ulrich Schwaneberg, Lutz Schmitt

Open access · hybridAbstract read
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In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 2 countries.

Zohreh Pourhassan NInstitute of Biochemistry, Heinrich Heine University, Universitätsstrasse 1, 40225, Düsseldorf, Germany.
Haiyang CuiInstitute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074, Aachen, Germany.ORCID 0000-0001-8360-0447
Sakshi KhosaInstitute of Biochemistry, Heinrich Heine University, Universitätsstrasse 1, 40225, Düsseldorf, Germany.
Mehdi D DavariDepartment of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120, Halle, Germany.
Karl-Erich JaegerInstitute of Molecular Enzyme Technology, Heinrich Heine University and Institute of Bio- and Geosciences IBG-1, Biotechnology, Forschungszentrum Jülich GmbH, 52426, Jülich, Germany.
Sander H J SmitsInstitute of Biochemistry, Heinrich Heine University, Universitätsstrasse 1, 40225, Düsseldorf, Germany.
Ulrich SchwanebergInstitute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074, Aachen, Germany.
Lutz SchmittInstitute of Biochemistry, Heinrich Heine University, Universitätsstrasse 1, 40225, Düsseldorf, Germany.ORCID 0000-0002-1167-9819
Heinrich Heine University Düsseldorf · DEDWI – Leibniz Institute for Interactive Materials · DEForschungszentrum Jülich · DELeibniz Institute of Plant Biochemistry · DEUniversity of Illinois Urbana-Champaign · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Type 1 secretion systems (T1SS) have a relatively simple architecture compared to other classes of secretion systems and therefore, are attractive to be optimized by protein engineering. Here, we report a KnowVolution campaign for the hemolysin (Hly) enhancer fragment, an untranslated region upstream of the hlyA gene, of the hemolysin T1SS of Escherichia coli to enhance its secretion efficiency. The best performing variant of the Hly enhancer fragment contained five nucleotide mutations at five positions (A30U, A36U, A54G, A81U, and A116U) resulted in a 2-fold increase in the secretion level of a model lipase fused to the secretion carrier HlyA1. Computational analysis suggested that altered affinity to the generated enhancer fragment towards the S1 ribosomal protein contributes to the enhanced secretion levels. Furthermore, we demonstrate that involving a native terminator region along with the generated Hly enhancer fragment increased the secretion levels of the Hly system up to 5-fold.

Indexed as

Escherichia coliEscherichia coli ProteinsHemolysin ProteinsProtein EngineeringTerminator Regions, GeneticType I Secretion SystemsEscherichia coli ProteinsHemolysin ProteinsType I Secretion Systemsdirected evolutionHly enhancer fragmentimproved secretion efficiencyproteinprotein secretion

Identifiers

PMID35062047
PMCPMC9306574
OpenAlexW4205224334

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.