ArticleeLife2022
Nanofluidic chips for cryo-EM structure determination from picoliter sample volumes.
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Microsecond Time-Resolved Cryo-EM Based on Jet Vitrification.bioRxiv : the preprint server for biology · 2025Article
- Site-directed tyrosinase conjugation on engineered ferritin retains immune recognition.Scientific reports · 2025Article
- Foam film vitrification for cryo-EM.Nature communications · 2025Article
- FLUID-CELL: Flow-enabled Light and Ultrastructural Imaging Device for Correlative Electron and Light Localization.bioRxiv : the preprint server for biology · 2025Article
- Advancing Cryo-EM and Cryo-ET through Innovation in Sample Carriers: A Perspective.Analytical chemistry · 2025Review
- A large-scale curated and filterable dataset for cryo-EM foundation model pre-training.Scientific data · 2025Article
- Illuminating ubiquitination mechanisms: How cryo-EM has shed light on Cullin RING E3 ligase function.Current opinion in structural biology · 2025Review
- Factors affecting macromolecule orientations in thin films formed in cryo-EM.Acta crystallographica. Section D, Structural biology · 2024Article
- CryoEM grid preparation: a closer look at advancements and impact of preparation mode and new approaches.Biochemical Society transactions · 2024Review
- Graphene sandwich-based biological specimen preparation for cryo-EM analysis.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Metallo-supramolecular branched polymer protects particles from air-water interface in single-particle cryo-electron microscopy.Communications biology · 2024Article
- Copper Oxide Spike Grids for Enhanced Solution Transfer in Cryogenic Electron Microscopy.Molecules and cells · 2023Article
- Cryo-EM samples of gas-phase purified protein assemblies using native electrospray ion-beam deposition.Faraday discussions · 2022Article
- Recent Technical Advances in Sample Preparation for Single-Particle Cryo-EM.Frontiers in molecular biosciences · 2022Review
- Perspective: Biochemical and Physical Constraints Associated With Preparing Thin Specimens for Single-Particle Cryo-EM.Frontiers in molecular biosciences · 2022Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryogenic electron microscopy has become an essential tool for structure determination of biological macromolecules. In practice, the difficulty to reliably prepare samples with uniform ice thickness still represents a barrier for routine high-resolution imaging and limits the current throughput of the technique. We show that a nanofluidic sample support with well-defined geometry can be used to prepare cryo-EM specimens with reproducible ice thickness from picoliter sample volumes. The sample solution is contained in electron-transparent nanochannels that provide uniform thickness gradients without further optimisation and eliminate the potentially destructive air-water interface. We demonstrate the possibility to perform high-resolution structure determination with three standard protein specimens. Nanofabricated sample supports bear potential to automate the cryo-EM workflow, and to explore new frontiers for cryo-EM applications such as time-resolved imaging and high-throughput screening.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.