Evidence map›Paper›PMID 35059744›Full record

ArticleMolecular medicine reports2022

CpG site hypomethylation at ETS1‑binding region regulates DLK1 expression in Chinese patients with Tetralogy of Fallot.

Guixiang Tian, Lili He, Ruoyi Gu, Jingwei Sun, Weicheng Chen, Yanyan Qian, Xiaojing Ma, Weili Yan, Zhenshan Zhao, Ziqing Xu and 3 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular medicine reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
0.3field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it, 4 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Guixiang Tian *Department of Ultrasound, Cardiovascular Center, Pediatrics Research Institute, National Children's Medical Center, Children's Hospital of Fudan University, Shanghai 201102, P.R. China.
Lili He *Department of Ultrasound, Cardiovascular Center, Pediatrics Research Institute, National Children's Medical Center, Children's Hospital of Fudan University, Shanghai 201102, P.R. China.
Ruoyi Gu *Department of Ultrasound, Cardiovascular Center, Pediatrics Research Institute, National Children's Medical Center, Children's Hospital of Fudan University, Shanghai 201102, P.R. China.
Jingwei SunPediatrics Department, Bengbu First People's Hospital, Bengbu, Anhui 233000, P.R. China.
Weicheng ChenDepartment of Ultrasound, Cardiovascular Center, Pediatrics Research Institute, National Children's Medical Center, Children's Hospital of Fudan University, Shanghai 201102, P.R. China.
Yanyan QianDepartment of Ultrasound, Cardiovascular Center, Pediatrics Research Institute, National Children's Medical Center, Children's Hospital of Fudan University, Shanghai 201102, P.R. China.
Xiaojing MaDepartment of Ultrasound, Cardiovascular Center, Pediatrics Research Institute, National Children's Medical Center, Children's Hospital of Fudan University, Shanghai 201102, P.R. China.
Weili YanDepartment of Ultrasound, Cardiovascular Center, Pediatrics Research Institute, National Children's Medical Center, Children's Hospital of Fudan University, Shanghai 201102, P.R. China.
Zhenshan ZhaoDepartment of Ultrasound, Cardiovascular Center, Pediatrics Research Institute, National Children's Medical Center, Children's Hospital of Fudan University, Shanghai 201102, P.R. China.
Ziqing XuDepartment of Ultrasound, Cardiovascular Center, Pediatrics Research Institute, National Children's Medical Center, Children's Hospital of Fudan University, Shanghai 201102, P.R. China.
Meijiao SuoDepartment of Ultrasound, Cardiovascular Center, Pediatrics Research Institute, National Children's Medical Center, Children's Hospital of Fudan University, Shanghai 201102, P.R. China.
Wei ShengDepartment of Ultrasound, Cardiovascular Center, Pediatrics Research Institute, National Children's Medical Center, Children's Hospital of Fudan University, Shanghai 201102, P.R. China.
Guoying HuangDepartment of Ultrasound, Cardiovascular Center, Pediatrics Research Institute, National Children's Medical Center, Children's Hospital of Fudan University, Shanghai 201102, P.R. China.
Children's Hospital of Fudan University · CNFirst Affiliated Hospital of Bengbu Medical College · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tetralogy of Fallot (TOF) is the most common cyanotic congenital heart malformation accounting for ~10% of cases. Although the pathogenesis of TOF is complex and largely unknown, epigenetics plays a huge role, specifically DNA methylation. The protein δ like non‑canonical Notch ligand 1 (DLK1) gene encodes a non‑canonical ligand of the Notch signaling pathway, which is involved in heart development. However, the epigenetic mechanism of DLK1 in the pathogenesis of TOF is yet to be elucidated. Therefore, the present study aimed to clarify its specific mechanism. In this study, immunohistochemistry was used to detect the protein expression of DLK1 and the methylation status of the DLK1 promoter was measured via bisulfite sequencing PCR. Dual‑luciferase reporter assays were performed to examine the influence of transcription factor ETS proto‑oncogene 1 (ETS1) on DLK1 gene expression. The electrophoretic mobility shift assay and chromatin immunoprecipitation assay, both

Indexed as

DNA MethylationGene Expression RegulationAsian PeopleBase SequenceBinding SitesCalcium-Binding ProteinsChild, PreschoolChinaCpG IslandsFemaleHEK293 CellsHumansInfantMaleMembrane ProteinsPromoter Regions, GeneticCalcium-Binding ProteinsDLK1 protein, humanMembrane ProteinsProto-Oncogene Protein c-ets-1DNA methylationETS proto‑oncogene 1Tetralogy of Fallotδ like non‑canonical Notch ligand 1

Identifiers

PMID35059744
PMCPMC8809049
OpenAlexW4205745360

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.