Evidence map›Paper›PMID 35059653›Full record

ArticleSTAR protocols2022

A kinetic intra-cellular assay (KICA) to measure quantitative compound binding kinetics within living cells.

Charles S Lay, Daniel A Thomas, John P Evans, Emma J Jones, Kelly M Gatfield, Peter D Craggs

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Charles S LayDivision of Physiology, Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK.
Daniel A ThomasMedicine Design, Medicinal Science and Technology, GlaxoSmithKline, Stevenage SG1 2NY, UK.
John P EvansMedicine Design, Medicinal Science and Technology, GlaxoSmithKline, Stevenage SG1 2NY, UK.
Emma J JonesProtein and Cellular Sciences, Medicinal Science and Technology, GlaxoSmithKline, Stevenage SG1 2NY, UK.
Kelly M GatfieldMedicine Design, Medicinal Science and Technology, GlaxoSmithKline, Stevenage SG1 2NY, UK.
Peter D CraggsMedicine Design, Medicinal Science and Technology, GlaxoSmithKline, Stevenage SG1 2NY, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Kinetic Intra-Cellular Assay (KICA) is a recombinant cell-based technique that utilizes NanoBRET technology. KICA enables the measurement of intracellular binding kinetics. This protocol describes steps for cellular transfection and expression, followed by addition of a target specific fluorophore conjugated probe and a range of concentrations of competitor compounds, followed by the measurement of BRET in a 384 well format. Fitting the BRET data allows measurement of forward and reverse binding rates and the determination of

Indexed as

Fluorescent DyesKineticsFluorescent DyesCell-based AssaysCell BiologyHigh Throughput ScreeningMolecular/Chemical Probes

Identifiers

PMID35059653
PMCPMC8760444

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.