ReviewBiophysical reviews2021
Cryogenic superresolution correlative light and electron microscopy on the frontier of subcellular imaging.
Review in Biophysical reviews, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed.
- Bridging structural biology and clinical research through in-tissue cryo-electron tomography.The EMBO journal · 2024Article
- scNodes: a correlation and processing toolkit for super-resolution fluorescence and electron microscopy.Nature methods · 2023Article
- Precise targeting for 3D cryo-correlative light and electron microscopy volume imaging of tissues using a FinderTOP.Communications biology · 2023Article
- HOPE-SIM, a cryo-structured illumination fluorescence microscopy system for accurately targeted cryo-electron tomography.Communications biology · 2023Article
- Metallic support films reduce optical heating in cryogenic correlative light and electron tomography.Journal of structural biology · 2022Article
- Cryogenic superresolution correlative light and electron microscopy of vitreous sections.Biophysics reports · 2022Article
- Editors' Roundup: June 2022.Biophysical reviews · 2022Article
- Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electron microscopy (EM) reveals cellular ultrastructure at high definition but faces the challenges of identification of specific subcellular structures and localization of specific macromolecules, whereas fluorescence microscopy (FM) can label and localize specific molecules in cells. Correlative light and electron microscopy (CLEM) combines the advantages of both microscopic techniques. Imaging vitreous hydrated samples at cryogenic temperatures using CLEM enables observations of cellular components of interest and their cellular context in a near-native state. This cryo-CLEM approach is further strengthened by incorporation of superresolution fluorescence microscopy, which can precisely pinpoint targets on electron micrographs. Cryogenic superresolution correlative light and electron microscopy (csCLEM) is an emerging and promising imaging technique that is expected to unveil its full power in ultrastructural studies. The present review describes the logic and principles behind this technique, how the method is implemented, the prospects, and the challenges.
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Registered trials
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