ArticleSLAS technology2022
Sorting single-cell microcarriers using commercial flow cytometers.
Article in SLAS technology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Defining Functional T Cell Receptor Repertoires With Nanovial-Based Screening.Immunological reviews · 2026Review
- Cell assays in microcompartments driven by droplet microfluidics and hydrogel technologies.Journal of biochemistry · 2026Review
- Microsystem technologies for accelerating the discovery and translation of immunotherapies.Nature reviews. Drug discovery · 2026Review
- Enhancing large particle recovery in high-throughput functional cell sorting through ΔBOP optimization.Scientific reports · 2025Article
- Design of buffer property for the new enrichment method of circulating tumor cell based on immunomagnetic-negative separation.Computational and structural biotechnology journal · 2024Article
- Essential Fluidics for a Flow Cytometer.Current protocols · 2024Article
- Function-first discovery of high affinity monoclonal antibodies using Nanovial-based plasma B cell screening.bioRxiv : the preprint server for biology · 2024Article
- Optimizing cell therapy by sorting cells with high extracellular vesicle secretion.Nature communications · 2024Article
- Lab on a Particle Technologies.Analytical chemistry · 2024Review
- Defining T cell receptor repertoires using nanovial-based binding and functional screening.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Associating growth factor secretions and transcriptomes of single cells in nanovials using SEC-seq.Nature nanotechnology · 2024Article
- Sorting Technology for Mesenchymal Stem Cells from a Single Tissue Source.Stem cell reviews and reports · 2024Review
- SEC-seq: association of molecular signatures with antibody secretion in thousands of single human plasma cells.Nature communications · 2023Article
- Optimizing Cell Therapy by Sorting Cells with High Extracellular Vesicle Secretion.bioRxiv : the preprint server for biology · 2023Article
- Integration of three non-interfering SERS probes combined with ConA-functionalized magnetic nanoparticles for extraction and detection of multiple foodborne pathogens.Mikrochimica acta · 2023Article
- Defining T cell receptor repertoires using nanovial-based affinity and functional screening.bioRxiv : the preprint server for biology · 2023Article
- Secretion encoded single-cell sequencing (SEC-seq) uncovers gene expression signatures associated with high VEGF-A secretion in mesenchymal stromal cells.bioRxiv : the preprint server for biology · 2023Article
- Suspendable Hydrogel Nanovials for Massively Parallel Single-Cell Functional Analysis and Sorting.ACS nano · 2022Article
- Single-cell sorting based on secreted products for functionally defined cell therapies.Microsystems & nanoengineering · 2022Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The scale of biological discovery is driven by the vessels in which we can perform assays and analyze results, from multi-well plates to microfluidic compartments. We report on the compatibility of sub-nanoliter single-cell containers or "nanovials" with commercial fluorescence activated cell sorters (FACS). This recent lab on a particle approach utilizes 3D structured microparticles to isolate cells and perform single-cell assays at scale with existing lab equipment. Use of flow cytometry led to detection of fluorescently labeled protein with dynamic ranges spanning 2-3 log and detection limits down to ∼10,000 molecules per nanovial, which was the lowest amount tested. Detection limits were improved compared to fluorescence microscopy measurements using a 20X objective and a cooled CMOS camera. Nanovials with diameters between 35-85 µm could also be sorted with purity from 99-93% on different commercial instruments at throughputs up to 800 events/second. Cell-loaded nanovials were found to have unique forward and side (or back) scatter signatures that enabled gating of cell-containing nanovials using scatter metrics alone. The compatibility of nanovials with widely-available commercial FACS instruments promises to democratize single-cell assays used in discovery of antibodies and cell therapies, by enabling analysis of single cells based on secreted products and leveraging the unmatched analytical capabilities of flow cytometers to sort important clones.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.