Evidence map›Paper›PMID 35057862›Full record

ArticleStem cell research & therapy2022

Extracellular vesicles from adipose-derived stem cells promote microglia M2 polarization and neurological recovery in a mouse model of transient middle cerebral artery occlusion.

Xiaowen Hu, Jiaji Pan, Yongfang Li, Yixu Jiang, Haoran Zheng, Rubing Shi, Qi Zhang, Chang Liu, Hengli Tian, Zhijun Zhang and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed, 2 pooled it
4.0field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 2 syntheses or guidelines pooled it, 51 citations in OpenAlex.

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  15. Emerging Roles of Exosomes in Stroke Therapy.International journal of molecular sciences · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 1 institution in 1 country.

Xiaowen HuShanghai Sixth People's Hospital and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
Jiaji PanShanghai Sixth People's Hospital and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
Yongfang LiDepartment of Rehabilitation and Institute of Neurology, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China.
Yixu JiangShanghai Sixth People's Hospital and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
Haoran ZhengShanghai Sixth People's Hospital and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
Rubing ShiShanghai Sixth People's Hospital and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
Qi ZhangShanghai Sixth People's Hospital and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
Chang LiuShanghai Sixth People's Hospital and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
Hengli TianShanghai Sixth People's Hospital and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
Zhijun ZhangShanghai Sixth People's Hospital and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
Yaohui TangShanghai Sixth People's Hospital and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
Guo-Yuan YangShanghai Sixth People's Hospital and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China. gyyang@sjtu.edu.cn.
Yongting WangShanghai Sixth People's Hospital and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China. ytwang@sjtu.edu.cn.ORCID 0000-0003-3105-9307
Shanghai Jiao Tong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAdipose-derived stem cells (ADSCs) and their extracellular vesicles (EVs) have therapeutic potential in ischemic brain injury, but the underlying mechanism is poorly understood. The current study aimed to explore the contribution of miRNAs in ADSC-EVs to the treatment of cerebral ischemia.

methodsAfter the intravenous injection of ADSC-EVs, therapeutic efficacy was evaluated by neurobehavioral tests and brain atrophy volume. The polarization of microglia was assessed by immunostaining and qPCR. We further performed miRNA sequencing of ADSC-EVs and analyzed the relationship between the upregulated miRNAs in ADSC-EVs and microglial polarization-related proteins using Ingenuity Pathway Analysis (IPA).

resultsThe results showed that ADSC-EVs reduced brain atrophy volume, improved neuromotor and cognitive functions after mouse ischemic stroke. The loss of oligodendrocytes was attenuated after ADSC-EVs injection. The number of blood vessels, as well as newly proliferated endothelial cells in the peri-ischemia area were higher in the ADSC-EVs treated group than that in the PBS group. In addition, ADSC-EVs regulated the polarization of microglia, resulting in increased repair-promoting M2 phenotype and decreased pro-inflammatory M1 phenotype. Finally, STAT1 and PTEN were highlighted as two downstream targets of up-regulated miRNAs in ADSC-EVs among 85 microglia/macrophage polarization related proteins by IPA. The inhibition of STAT1 and PTEN by ADSC-EVs were confirmed in cultured microglia.

conclusionsIn summary, ADSC-EVs reduced ischemic brain injury, which was associated with the regulation of microglial polarization. miRNAs in ADSC-EVs partly contributed to their function in regulating microglial polarization by targeting PTEN and STAT1.

Indexed as

Brain IschemiaExtracellular VesiclesAnimalsCells, CulturedEndothelial CellsInfarction, Middle Cerebral ArteryMiceMicrogliaStem CellsAdipose-derived stem cellCerebral ischemiaExtracellular vesiclesMicroglial polarizationmiRNAs

Identifiers

PMID35057862
PMCPMC8772170
OpenAlexW4206159027

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.