ArticleMicrobiome2022
Investigation of fiber utilization in the rumen of dairy cows based on metagenome-assembled genomes and single-cell RNA sequencing.
Article in Microbiome, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 92 citations in OpenAlex.
- Research progress of Rumen microbes utilizing lignocellulose for high-value chemical production.Biodesign research · 2026Review
- Subacute ruminal acidosis in high-producing dairy cows: an integrated diet-microbiota-epithelium-host framework for diagnosis and nutritional management.Journal of animal science and biotechnology · 2026Review
- Host-microbial co-regulation mediates rumen volatile fatty acid production and utilization in dairy cows.Science China. Life sciences · 2026Article
- Rumen Mycobiome Thiamine Metabolism Contributes to Subacute Rumen Acidosis Tolerance in Goats Through Enhancing Epithelial Cell Proliferation via IGFBP2/IGF1 Axis Activation.Exploration (Beijing, China) · 2026Article
- Production, Transport, and Metabolism of Volatile Fatty Acids in the Yak Rumen: Unraveling the Unique Mechanisms Underpinning High-Altitude Adaptation.Microorganisms · 2026Review
- Association of the residual feed intake (RFI) with the rumen microbiota composition and metabolism in Dorper-Hu crossbred lambs.BMC microbiology · 2026Article
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- BileActome reveals community-assembled bile acid metabolism in the rumen microbiome.ISME communications · 2026Article
- Microbiome-host co-oscillation patterns in shaping ruminal ecosystem from birth to puberty in a goat model.Science China. Life sciences · 2026Article
- Article
- Multiomics insights into rumen microbiome and function in grazing lambs: implications for nutrient absorption and grassland sustainability.Microbiome · 2025Article
- Identification of endoglucanase-encoding genes from goat rumen bacterial metagenomes using homology- and motif-based approaches.Archives of microbiology · 2025Article
- Red seaweed supplementation suppresses methanogenesis in the rumen, revealing potentially advantageous traits among hydrogenotrophic bacteria.Microbiome · 2025Article
- Ruminal dysbiosis-induced mastitis: new insight into the pathogenesis of mastitis.Journal of animal science and biotechnology · 2025Review
- Multi-omics insights into microbiome-rumen epithelium interaction mechanisms underlying subacute rumen acidosis tolerance in dairy goats.Genome biology · 2025Article
- A multi-tissue single-cell expression atlas in cattle.Nature genetics · 2025Article
- Effects of Partial Replacement of Alfalfa Hay with Alfalfa Silage in Dairy Cows: Impacts on Production Performance and Rumen Microbiota.Animals : an open access journal from MDPI · 2025Article
- Host Genetic Effects and Phenotypic Landscapes of Rumen Bacterial Enterotypes in a Large Sheep Population.Animals : an open access journal from MDPI · 2025Article
- Understanding the differences in rumen bacteria and their impact on dairy cows' production performance: A review.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDairy cows utilize human-inedible, low-value plant biomass to produce milk, a low-cost product with rich nutrients and high proteins. This process largely relies on rumen microbes that ferment lignocellulose and cellulose to produce volatile fatty acids (VFAs). The VFAs are absorbed and partly metabolized by the stratified squamous rumen epithelium, which is mediated by diverse cell types. Here, we applied a metagenomic binning approach to explore the individual microbes involved in fiber digestion and performed single-cell RNA sequencing on rumen epithelial cells to investigate the cell subtypes contributing to VFA absorption and metabolism.
resultsThe 52 mid-lactating dairy cows in our study (parity = 2.62 ± 0.91) had milk yield of 33.10 ± 6.72 kg. We determined the fiber digestion and fermentation capacities of 186 bacterial genomes using metagenomic binning and identified specific bacterial genomes with strong cellulose/xylan/pectin degradation capabilities that were highly associated with the biosynthesis of VFAs. Furthermore, we constructed a rumen epithelial single-cell map consisting of 18 rumen epithelial cell subtypes based on the transcriptome of 20,728 individual epithelial cells. A systematic survey of the expression profiles of genes encoding candidates for VFA transporters revealed that IGFBP5
conclusionsWe discovered the key individual microbial genomes and epithelial cell subtypes involved in fiber digestion, VFA uptake and metabolism, respectively, in the rumen. The integration of these data enables us to link microbial genomes and epithelial single cells to the trophic system. Video abstract.
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