ArticleACS synthetic biology2022
Cell-Free Gene Expression Dynamics in Synthetic Cell Populations.
Article in ACS synthetic biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Automated synthetic cell-based screening for designed proteins with emergent functions.Nature communications · 2026Article
- QuantGUV: Quantifying Encapsulation Efficiency of Small Molecules in Giant Unilamellar Vesicles.ACS applied materials & interfaces · 2026Article
- Image-based phenotypic sorting of synthetic cells.Science advances · 2026Article
- Absolute quantification of fluorescent protein fusions by mass spectrometry.Protein science : a publication of the Protein Society · 2026Article
- Riboswitch-controlled lipid conversion enables functional membrane asymmetry in artificial cells.Communications biology · 2026Article
- Bottom-up Strategies for Generating Polymer Protocells That Mimic Cellular Communication.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Review
- Characterizing Cell-Free Transcription and Translation Dynamics with Nucleic Acid-Based Assays.ACS synthetic biology · 2026Article
- Combining Nanoscale Curvature and Polymer Osmotic Pressure for Efficient Giant Vesicle Assembly Under Physiological Conditions.Small methods · 2025Article
- Artificial cells with liquid-liquid phase separation-regulated cell-free protein synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Cell-free protein synthesis and vesicle systems for programmable therapeutic manufacturing and delivery.Journal of biological engineering · 2025Review
- Concentration-Dependent CsrA Regulation of theACS synthetic biology · 2025Article
- On-the-Fly Microfluidic Control of Giant Vesicle Compositions Validated by DNA Surface Charge Sensors.ACS nano · 2025Article
- Simultaneous Detection of Multiple Analytes at Ambient Temperature Using Eukaryotic Artificial Cells with Modular and Robust Synthetic Riboswitches.ACS synthetic biology · 2025Article
- A mathematical model of cell-free transcription-translation with plasmid crosstalk.Synthetic biology (Oxford, England) · 2025Article
- Synthetic cells for phage therapy: a perspective.Frontiers in cellular and infection microbiology · 2025Review
- Regulatory Components for Bacterial Cell-Free Systems Engineering.ACS synthetic biology · 2024Review
- Large-scale-integration and collective oscillations of 2D artificial cells.Nature communications · 2024Article
- Genetically programmed synthetic cells for thermo-responsive protein synthesis and cargo release.Nature chemical biology · 2024Article
- Complex Emulsions as an Innovative Pharmaceutical Dosage form in Addressing the Issues of Multi-Drug Therapy and Polypharmacy Challenges.Pharmaceutics · 2024Review
- Models of Chemical Communication for Micro/Nanoparticles.Accounts of chemical research · 2024Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to build synthetic cellular populations from the bottom-up provides the groundwork to realize minimal living tissues comprising single cells which can communicate and bridge scales into multicellular systems. Engineered systems made of synthetic micron-sized compartments and integrated reaction networks coupled with mathematical modeling can facilitate the design and construction of complex and multiscale chemical systems from the bottom-up. Toward this goal, we generated populations of monodisperse liposomes encapsulating cell-free expression systems (CFESs) using double-emulsion microfluidics and quantified transcription and translation dynamics within individual synthetic cells of the population using a fluorescent Broccoli RNA aptamer and mCherry protein reporter. CFE dynamics in bulk reactions were used to test different coarse-grained resource-limited gene expression models using model selection to obtain transcription and translation rate parameters by likelihood-based parameter estimation. The selected model was then applied to quantify cell-free gene expression dynamics in populations of synthetic cells. In combination, our experimental and theoretical approaches provide a statistically robust analysis of CFE dynamics in bulk and monodisperse synthetic cell populations. We demonstrate that compartmentalization of CFESs leads to different transcription and translation rates compared to bulk CFE and show that this is due to the semipermeable lipid membrane that allows the exchange of materials between the synthetic cells and the external environment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.