Evidence map›Paper›PMID 35056718›Full record

ArticleMolecules (Basel, Switzerland)2022

Liposome-Mediated Delivery of MERS Antigen Induces Potent Humoral and Cell-Mediated Immune Response in Mice.

Masood Alam Khan, Ajamaluddin Malik, Mohammad A Alzohairy, Abdulmohsen M Alruwetei, Bader Y Alhatlani, Osamah Al Rugaie, Arif Khan

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Masood Alam KhanDepartment of Basic Health Sciences, College of Applied Medical Sciences, Qassim University, Buraydah 51452, Saudi Arabia.ORCID 0000-0003-4031-4189
Ajamaluddin MalikDepartment of Biochemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Mohammad A AlzohairyDepartment of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah 51452, Saudi Arabia.
Abdulmohsen M AlruweteiDepartment of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah 51452, Saudi Arabia.ORCID 0000-0001-8472-0440
Bader Y AlhatlaniDepartment of Applied Medical Sciences, Applied College in Unayzah, Qassim University, Unayzah 51911, Saudi Arabia.ORCID 0000-0003-0871-6313
Osamah Al RugaieDepartment of Basic Medical Sciences, College of Medicine and Medical Sciences, Qassim University, Unayzah 51911, Saudi Arabia.ORCID 0000-0001-8148-0138
Arif KhanDepartment of Basic Health Sciences, College of Applied Medical Sciences, Qassim University, Buraydah 51452, Saudi Arabia.ORCID 0000-0002-4528-2668
Qassim University · SAKing Saud University · SA

Funding

Deanship of Scientific Research, Qassim University, Buraydah, Saudi Arabia 5575-cams1-2019-2-2-I
6 · The paper itself

Abstract

The advancements in the field of nanotechnology have provided a great platform for the development of effective antiviral vaccines. Liposome-mediated delivery of antigens has been shown to induce the antigen-specific stimulation of the humoral and cell-mediated immune responses. Here, we prepared dried, reconstituted vesicles (DRVs) from DPPC liposomes and used them as the vaccine carrier system for the Middle East respiratory syndrome coronavirus papain-like protease (DRVs-MERS-CoV PLpro). MERS-CoV PLpro emulsified in the Incomplete Freund's Adjuvant (IFA-MERS-CoV PLpro) was used as a control. Immunization of mice with DRVs-MERS-CoV PLpro did not induce any notable toxicity, as revealed by the levels of the serum alanine transaminase (ALT), aspartate transaminase (AST), blood urea nitrogen (BUN) and lactate dehydrogenase (LDH) in the blood of immunized mice. Immunization with DRVs-MERS-CoV PLpro induced greater antigen-specific antibody titer and switching of IgG1 isotyping to IgG2a as compared to immunization with IFA-MERS-CoV PLpro. Moreover, splenocytes from mice immunized with DRVs-MERS-CoV PLpro exhibited greater proliferation in response to antigen stimulation. Moreover, splenocytes from DRVs-MERS-CoV PLpro-immunized mice secreted significantly higher IFN-γ as compared to splenocytes from IFA-MERS-CoV PLpro mice. In summary, DRVs-MERS-CoV PLpro may prove to be an effective prophylactic formulation to prevent MERS-CoV infection.

Indexed as

AnimalsCell ProliferationCoronavirus InfectionsCoronavirus Papain-Like ProteasesFemaleImmunity, CellularImmunity, HumoralImmunizationImmunoglobulin GInterferon-gammaLiposomesLymphocytesMiceMiddle East Respiratory Syndrome CoronavirusViral VaccinesCoronavirus Papain-Like ProteasesImmunoglobulin GInterferon-gammaLiposomesViral Vaccinesantiviral immunityliposomeMERS-CoVvaccine delivery

Identifiers

PMID35056718
PMCPMC8778403
OpenAlexW4205871393

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.