Evidence map›Paper›PMID 35054953›Full record

ArticleInternational journal of molecular sciences2022

Multiphoton Bleaching of Red Fluorescent Proteins and the Ways to Reduce It.

Mikhail Drobizhev, Rosana S Molina, Jacob Franklin

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.6field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Comparing confocal and two-photon CaBiophysical reports · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Mikhail DrobizhevDepartment of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717, USA.
Rosana S MolinaDepartment of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717, USA.
Jacob FranklinVidrio Technologies LLC, 19955 Highland Vista Drive Suite 150, Ashburn, VA 20147, USA.
Montana State University · USVidrio (United States) · US

Funding

Resource for Multiphoton Characterization of Genetically-Encoded ProbesU24NS109107 · NINDS · MONTANA STATE UNIVERSITY - BOZEMAN · PI Mikhail Drobizhev · 2018 to 2026
$2.2M
Northern Lights collaboration for better 2-photon probesU01NS094246 · NINDS · MONTANA STATE UNIVERSITY - BOZEMAN · PI CAMPBELL, ROBERT E., DROBIZHEV, MIKHAIL · 2015 to 2017
$2.0M
NIH HHS U01 NS094246NIH HHS U24 NS109107NINDS NIH HHS U01 NS094246NINDS NIH HHS U24 NS109107
6 · The paper itself

Abstract

Red fluorescent proteins and biosensors built upon them are potentially beneficial for two-photon laser microscopy (TPLM) because they can image deeper layers of tissue, compared to green fluorescent proteins. However, some publications report on their very fast photobleaching, especially upon excitation at 750-800 nm. Here we study the multiphoton bleaching properties of mCherry, mPlum, tdTomato, and jREX-GECO1, measuring power dependences of photobleaching rates

Indexed as

LasersMicroscopy, Fluorescence, MultiphotonPhotobleachingAlgorithmsHumansLuminescent ProteinsModels, TheoreticalRed Fluorescent ProteinLuminescent ProteinsRed Fluorescent Proteinelectron photodetachmentjREX-GECO1mCherrymPlummultiphoton absorptionmultiphoton bleachingphotoionizationred fluorescent proteinstdTomatotwo-photon laser microscopy

Identifiers

PMID35054953
PMCPMC8775990
OpenAlexW4205520802

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.