Evidence map›Paper›PMID 35054463›Full record

ArticleLife (Basel, Switzerland)2022

Evaluation of Three Automated Extraction Systems for the Detection of SARS-CoV-2 from Clinical Respiratory Specimens.

Ho-Jae Lim, Hye-Soo Jung, Min-Young Park, Young-Hyun Baek, Balaji Kannappan, Jin-Young Park, Jae-Hyun Yang, Ja-Hwan Seol, Min-Woo Lee, Sun-Kyung Jung and 3 more

Abstract read
In one paragraph

Article in Life (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Article
  2. Virology Applications to the COVID-19 Pandemic.Life (Basel, Switzerland) · 2025
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Evaluation of Alternative Transport Media for RT-qPCR-Based SARS-CoV-2 Testing.International journal of analytical chemistry · 2022
    Article
  14. Observational
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Ho-Jae LimDepartment of Molecular Diagnostics, Seegene Medical Foundation, Seoul 04805, Korea.ORCID 0000-0003-0115-5170
Hye-Soo JungDepartment of Molecular Diagnostics, Seegene Medical Foundation, Seoul 04805, Korea.ORCID 0000-0002-0900-2913
Min-Young ParkDepartment of Molecular Diagnostics, Seegene Medical Foundation, Seoul 04805, Korea.ORCID 0000-0002-5319-5644
Young-Hyun BaekDepartment of Molecular Diagnostics, Seegene Medical Foundation, Seoul 04805, Korea.ORCID 0000-0002-6894-9293
Balaji KannappanBK21 FOUR Educational Research Group for Age-Associated Disorder Control Technology, Department of Integrative Biological Sciences, Chosun University, Gwangju 61452, Korea.
Jin-Young ParkDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Jae-Hyun YangPaul F. Glenn Center for Biology of Aging Research, Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA.
Ja-Hwan SeolDepartment of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA.
Min-Woo LeeDepartment of Integrated Biomedical Science, Soonchunhyang Institute of Medi-Bio Science (SIMS), Soonchunhyang University, Cheonan-si 31151, Korea.
Sun-Kyung JungDepartment of Molecular Diagnostics, Seegene Medical Foundation, Seoul 04805, Korea.
Sun-Hwa LeeDepartment of Molecular Diagnostics, Seegene Medical Foundation, Seoul 04805, Korea.
Jung-Eun ParkBK21 FOUR Educational Research Group for Age-Associated Disorder Control Technology, Department of Integrative Biological Sciences, Chosun University, Gwangju 61452, Korea.ORCID 0000-0001-8789-9829
Yong-Jin YangDepartment of Molecular Diagnostics, Seegene Medical Foundation, Seoul 04805, Korea.ORCID 0000-0002-6153-9646

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Severe acute respiratory syndrome coronavirus (SARS-CoV-2) is highly contagious and causes coronavirus disease 2019 (COVID-19). Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is the most accurate and reliable molecular assay to detect active SARS-CoV-2 infection. However, a rapid increase in test subjects has created a global bottleneck in testing capacity. Given that efficient nucleic acid extraction greatly affects reliable and accurate testing results, we compared three extraction platforms: MagNA Pure 96 DNA and Viral NA Small Volume kit on MagNA Pure 96 (Roche, Basel, Switzerland), careGENE

Indexed as

automated extraction systemmRT-qPCRSARS-CoV-2

Identifiers

PMID35054463
PMCPMC8777774

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.