Evidence map›Paper›PMID 35054213›Full record

ArticleDiagnostics (Basel, Switzerland)2021

Detection of Microorganisms in Body Fluids via MTT-PMS Assay.

Cheng-Han Chen, Yu-Ting Tsao, Po-Ting Yeh, Yu-Hsiang Liao, Yi-Tzu Lee, Wan-Ting Liao, Yung-Chih Wang, Ching-Fen Shen, Chao-Min Cheng

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diagnostics (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 1 country.

Cheng-Han ChenInstitute of Biomedical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.ORCID 0000-0002-9542-525X
Yu-Ting TsaoInstitute of Biomedical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.
Po-Ting YehDepartment of Ophthalmology, National Taiwan University Hospital, Taipei 10002, Taiwan.ORCID 0000-0002-0461-8778
Yu-Hsiang LiaoInstitute of Biomedical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.
Yi-Tzu LeeDepartment of Emergency Medicine, Taipei Veterans General Hospital, Taipei 11217, Taiwan.ORCID 0000-0002-6183-4987
Wan-Ting LiaoInstitute of Biomedical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.
Yung-Chih WangNational Defense Medical Center, Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, Taipei 11490, Taiwan.
Ching-Fen ShenDepartment of Pediatrics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan.ORCID 0000-0001-5926-0462
Chao-Min ChengInstitute of Biomedical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.ORCID 0000-0002-8644-1960
National Tsing Hua University · TWNational Yang Ming Chiao Tung University · TWNational Cheng Kung University Hospital · TWNational Taiwan University Hospital · TWTri-Service General Hospital · TW

Funding

the Frontier Research Center on Fundamental and Applied Sciences of Matters from the Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education, Taiwan MOE 107QR001I5the Ministry of Science and Technology, Taiwan MOST 107-2628-E-007-001-MY3, MOST 110-2221-E-007-073-MY2, MOST 109-2222-E-075-001, MOST 109-2635-B-006-004, and MOST 110-2923-B-006-001-MY4
6 · The paper itself

Abstract

Early detection of microorganisms is essential for the management of infectious diseases. However, this is challenging, as traditional culture methods are labor-intensive and time-consuming. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-phenazine methosulfate (MTT-PMS) assay has been used to evaluate the metabolic activity in live cells and can thus be used for detecting living microorganisms. With the addition of NaOH and Tris-EDTA, the same approach can be accelerated (within 15 min) and used for the quick detection of common bacterial pathogens. The assay results can be evaluated colorimetrically or semi-quantitatively. Here, the quick detection by MTT-PMS assay was further investigated. The assay had a detection limit of approximately 10

Indexed as

bacterial detectionhuman body fluidsinfectious diseasesMTT-PMS assaypoint-of-care testing

Identifiers

PMID35054213
PMCPMC8774610
OpenAlexW4200003892

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.