Evidence map›Paper›PMID 35052988›Full record

ArticleAntibiotics (Basel, Switzerland)2022

Optimization of Propidium Monoazide qPCR (Viability-qPCR) to Quantify the Killing by the

Agnieszka Latka, Leen Van Simaey, Marijke Reynders, Piet Cools, Tess Rogier, Barbara Lebbe, Lorenzo Corsini, Christine Landlinger, Mario Vaneechoutte

Abstract read
In one paragraph

Article in Antibiotics (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Optimization ofJournal of environmental engineering (New York, N.Y.) · 2025
    Article
  6. PeerJ · 2025
    Article
  7. Article
  8. Prevalence and clinical correlates ofFrontiers in cellular and infection microbiology · 2024
    Article
  9. Article
  10. Biofilm · 2023
    Article
  11. Article
  12. Six Bacterial Vaginosis-Associated Species Can Form anFrontiers in cellular and infection microbiology · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Agnieszka LatkaLaboratory Bacteriology Research, Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium.ORCID 0000-0003-4492-497X
Leen Van SimaeyLaboratory Bacteriology Research, Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium.
Marijke ReyndersLaboratory Medicine, Medical Microbiology, AZ St.Jan Brugge-Oostende AV, 8000 Bruges, Belgium.ORCID 0000-0003-2079-1279
Piet CoolsLaboratory Bacteriology Research, Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium.ORCID 0000-0003-2980-5307
Tess RogierLaboratory Bacteriology Research, Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium.ORCID 0000-0003-1966-5144
Barbara LebbeDepartment of Gynaecology, AZ St.Jan Brugge-Oostende AV, 8000 Bruges, Belgium.ORCID 0000-0002-8671-878X
Lorenzo CorsiniPhagoMed Biopharma GmbH, Vienna Biocenter, 1110 Wien, Austria.ORCID 0000-0002-8077-6559
Christine LandlingerPhagoMed Biopharma GmbH, Vienna Biocenter, 1110 Wien, Austria.
Mario VaneechoutteLaboratory Bacteriology Research, Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Quantification of the number of living cells in biofilm or after eradication treatments of biofilm, is problematic for different reasons. We assessed the performance of pre-treatment of DNA, planktonic cells and ex vivo vaginal biofilms of

Indexed as

bacterial vaginosisbiofilmendolysinGardnerellaviability-qPCR

Identifiers

PMID35052988
PMCPMC8773202

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.