Evidence map›Paper›PMID 35052471›Full record

ArticleGenes2022

Diane-35 and Metformin Induce Autophagy and Apoptosis in Polycystic Ovary Syndrome Women with Early-Stage Endometrial Carcinoma.

Yanjun Liu, Yang Wang, Dan Yao, Xing Chen, Feifei Zhang, Yi Feng, Xin Li

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genes, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Yanjun LiuDepartment of Obstetrics and Gynecology, Shanghai Medical School, Fudan University, Shanghai 200011, China.
Yang WangDepartment of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, China.ORCID 0000-0002-2830-2524
Dan YaoDepartment of Endocrinology, Xiangshan Hospital of TCM Medical and Health Group, Ningbo 315700, China.
Xing ChenDepartment of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, China.
Feifei ZhangDepartment of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, China.
Yi FengDepartment of Integrative Medicine and Neurobiology, School of Basic Medical Sciences, Institutes of Brain Science, Brain Science Collaborative Innovation Center, State Key Laboratory of Medical Neurobiology, Fudan Institutes of Integrative, Shanghai 200011, China.
Xin LiDepartment of Obstetrics and Gynecology, Shanghai Medical School, Fudan University, Shanghai 200011, China.
Obstetrics and Gynecology Hospital of Fudan University · CNShanghai Medical College of Fudan University · CNState Key Laboratory of Medical Neurobiology

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveWomen with polycystic ovary syndrome (PCOS) are at increased risk ofendometrial carcinoma (EC). Previous studies indicated that the combined therapy of Diane-35 and metformin significantly suppresses disease progression in PCOS patients with early EC; however, the mechanisms remain unclear.

methodsAn established murine model of PCOS with early EC, clinical specimens, and human EC cells was used in this study. The levels of protein and mRNA were measured with Western blotting and RT-PCR, respectively. Cell proliferation was determined with MTT, colony formation, and flow cytometry. Proteins were analyzed with immunofluorescence and immunohistochemistry.

resultsDiane-35 and metformin significantly inhibited proliferative activity and promoted apoptosis in EC cells. Additionally, cell autophagy was induced by the combined therapy. Quantitive PCR revealed that Diane-35 and metformin decreased androgen receptor (AR) expression but elevated GLUT4 expression. AR was found to repress GLUT4 expression by binding to the promoter of GLUT4. Moreover, the combined treatment mediated the onset of cellular autophagy by regulating the mTORC pathway via the suppression of IGF-1 and inhibited the development of EC by the activation of the PI3K/mTORC pathway.

conclusionThe results and previous clinical evidence support the use of Diane-35 and metformin combination therapy for patients with PCOS and early EC.

Indexed as

ApoptosisAutophagyAndrogen AntagonistsAnimalsCyproterone AcetateDrug CombinationsDrug Therapy, CombinationEndometrial NeoplasmsEthinyl EstradiolFemaleGlucose Transporter Type 4HumansHypoglycemic AgentsInsulin-Like Growth Factor IMetforminMiceAndrogen AntagonistsCyproterone AcetateCyproterone acetate, ethinyl estradiol drug combinationDrug CombinationsEthinyl EstradiolGlucose Transporter Type 4Hypoglycemic AgentsInsulin-Like Growth Factor IMetforminReceptors, AndrogenautophagyDiane-35early-stage endometrial carcinomametforminpolycystic ovary syndrome

Identifiers

PMID35052471
PMCPMC8775133
OpenAlexW4206523844

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.