Evidence map›Paper›PMID 35051448›Full record

ArticleJournal of biotechnology2022

Design and characterization of a salicylic acid-inducible gene expression system for Jurkat cells.

Aarti Doshi, Irfan Bandey, Dmitry Nevozhay, Navin Varadarajan, Patrick C Cirino

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Aarti DoshiDept. of Biology and Biochemistry, University of Houston, Houston, TX, USA. Electronic address: adoshi@uh.edu.
Irfan BandeyDept. of Chemical and Biomolecular Engineering, University of Houston, Houston, TX, USA. Electronic address: ibandey@central.uh.edu.
Dmitry NevozhayDept. of Imaging Physics, The University of Texas MD Anderson Cancer Center, Houston, TX, USA; School of Biomedicine, Far Eastern Federal University, Vladivostok, Russia. Electronic address: dnevozhay@mdanderson.org.
Navin VaradarajanDept. of Chemical and Biomolecular Engineering, University of Houston, Houston, TX, USA. Electronic address: nvaradar@central.uh.edu.
Patrick C CirinoDept. of Biology and Biochemistry, University of Houston, Houston, TX, USA; Dept. of Chemical and Biomolecular Engineering, University of Houston, Houston, TX, USA. Electronic address: pccirino@central.uh.edu.
University of Houston · USThe University of Texas MD Anderson Cancer Center · US

Funding

Systematic, molecular level analysis of the Fc receptor ligation on antibody effector functionsU01AI148118 · NIAID · UNIVERSITY OF TEXAS AT AUSTIN · PI GEORGIOU, GEORGE GEORGIOU, VARADARAJAN, NAVIN · 2020 to 2024
$2.3M
MusIC: A multi-scale technology for integrating dynamic cellular function and molecular profilesR01GM143243 · NIGMS · UNIVERSITY OF HOUSTON · PI VARADARAJAN, NAVIN · 2021 to 2024
$1.8M
NIAID NIH HHS U01 AI148118NIGMS NIH HHS R01 GM143243
6 · The paper itself

Abstract

With continued progress in cell and gene therapies, there is an immediate need for exogenously tunable gene expression systems with safe and predictable behavior in specific human cell types. Here, we demonstrate the ability of the salicylic acid (SA)-inducible MarR repressor protein from Escherichia coli to regulate target gene expression in a human T lymphocyte cell line. Two lentiviral vectors, one encoding an enhanced green fluorescent protein (EGFP) reporter cassette and the other a repressor cassette, were sequentially transduced into Jurkat cells, using fluorescence-activated cell sorting (FACS) to isolate stable Jurkat progeny. As a result, EGFP expression was repressed by MarR and was inducible upon the addition of SA (~1.3 fold). This represents the first example of functional expression of bacterial MarR in mammalian cells, and opens the possibility for further development of regulated, SA-tunable gene expression system for T-cells.

Indexed as

Genetic VectorsLentivirusAnimalsGene ExpressionGreen Fluorescent ProteinsHumansJurkat CellsSalicylic AcidGreen Fluorescent ProteinsSalicylic AcidFACSGene repressionInducible promoterJurkat cellsLentiviral transductionTranscriptional factors

Identifiers

PMID35051448
PMCPMC9618363
OpenAlexW4206005965

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.