ArticleBioengineering (Basel, Switzerland)2022
Alginate Hydrogel Microtubes for Salivary Gland Cell Organization and Cavitation.
Article in Bioengineering (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Cryoelectrospun Elastin-Alginate Scaffolds Support In Vitro 3D Epithelial-Stromal Cocultures for Salivary Tissue Engineering.Gels (Basel, Switzerland) · 2025Article
- Expansion of functional human salivary acinar cell spheroids with reversible thermo-ionically crosslinked 3D hydrogels.International journal of oral science · 2025Article
- Cost-reduction strategy to culture patient derived bladder tumor organoids.Scientific reports · 2025Article
- In-tube micro-pyramidal silicon nanopore for inertial-kinetic sensing of single molecules.Nature communications · 2024Article
- Advancements and Challenges in Hydrogel Engineering for Regenerative Medicine.Gels (Basel, Switzerland) · 2024Review
- Recent Advances in Alginate-Based Hydrogels for Cell Transplantation Applications.Pharmaceutics · 2024Review
- Salivary Gland Bioengineering.Bioengineering (Basel, Switzerland) · 2023Review
- Microfluidic coaxial 3D bioprinting of cell-laden microfibers and microtubes for salivary gland tissue engineering.Biomaterials advances · 2023Article
- Matrix Degradability Contributes to the Development of Salivary Gland Progenitor Cells with Secretory Functions.ACS applied materials & interfaces · 2023Article
- Biofabrication, biochemical profiling, and in vitro applications of salivary gland decellularized matrices via magnetic bioassembly platforms.Cell and tissue research · 2023Article
- Alginate microfibers as therapeutic delivery scaffolds and tissue mimics.Experimental biology and medicine (Maywood, N.J.) · 2022Review
- Bioengineering in salivary gland regeneration.Journal of biomedical science · 2022Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Understanding the different regulatory functions of epithelial and mesenchymal cell types in salivary gland development and cellular organization is essential for proper organoid formation and salivary gland tissue regeneration. Here, we demonstrate a biocompatible platform using pre-formed alginate hydrogel microtubes to facilitate direct epithelial-mesenchymal cell interaction for 3D salivary gland cell organization, which allows for monitoring cellular organization while providing a protective barrier from cell-cluster loss during medium changes. Using mouse salivary gland ductal epithelial SIMS cells as the epithelial model cell type and NIH 3T3 fibroblasts or primary E16 salivary mesenchyme cells as the stromal model cell types, self-organization from epithelial-mesenchymal interaction was examined. We observed that epithelial and mesenchymal cells undergo aggregation on day 1, cavitation by day 4, and generation of an EpCAM-expressing epithelial cell layer as early as day 7 of the co-culture in hydrogel microtubes, demonstrating the utility of hydrogel microtubes to facilitate heterotypic cell-cell interactions to form cavitated organoids. Thus, pre-formed alginate microtubes are a promising co-culture method for further understanding epithelial and mesenchymal interaction during tissue morphogenesis and for future practical applications in regenerative medicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.