Evidence map›Paper›PMID 35048333›Full record

ArticleGenes & genomics2022

Identification and differential expression of microRNAs in Madin-Darby canine kidney cells with high and low tumorigenicities.

Jiamin Wang, Lixia Liu, Di Yang, Li Zhang, Ayimuguli Abudureyimu, Zilin Qiao, Zhongren Ma

Abstract read
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In one paragraph

Article in Genes & genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Jiamin WangState Key Laboratory of Biotherapy, Sichuan University, No. 17, Section 3, Renmin South Road, Chengdu, 610041, China.
Lixia LiuGansu Tech Innovation Center of Animal Cell, Biomedical Research Center, Northwest Minzu University, Northwest Village No.1, Chengguan District, Lanzhou, 730030, China.
Di YangGansu Tech Innovation Center of Animal Cell, Biomedical Research Center, Northwest Minzu University, Northwest Village No.1, Chengguan District, Lanzhou, 730030, China.
Li ZhangGansu Tech Innovation Center of Animal Cell, Biomedical Research Center, Northwest Minzu University, Northwest Village No.1, Chengguan District, Lanzhou, 730030, China.
Ayimuguli AbudureyimuGansu Tech Innovation Center of Animal Cell, Biomedical Research Center, Northwest Minzu University, Northwest Village No.1, Chengguan District, Lanzhou, 730030, China.
Zilin QiaoGansu Tech Innovation Center of Animal Cell, Biomedical Research Center, Northwest Minzu University, Northwest Village No.1, Chengguan District, Lanzhou, 730030, China.
Zhongren MaState Key Laboratory of Biotherapy, Sichuan University, No. 17, Section 3, Renmin South Road, Chengdu, 610041, China. mzr@xbmu.edu.cn.
Minzu University of China · CNGansu Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMadin-Darby canine kidney (MDCK) cells are widely used for vaccine production, however, the safety of MDCK cells needs to be considered seriously because of high tumorigenicity. Micro RNAs (miRNAs) that are involved in the tumorigenicity of MDCK cells have been never been reported.

objectiveTo reveal the role of miRNA in the tumorigenic phenotype of MDCK cell line.

methodsThe miRNA expression profiles of two monoclonal MDCK cells (M09CL and M35CL) with low tumorigenicity and one MDCK cell line (M73P) with high tumorigenicity were characterized and investigated by using small RNA-seq technology.

resultsA total of 5 known miRNAs and 5 novel miRNAs were highly expressed in M73P. In addition, 4 known miRNAs and 4 novel miRNAs were highly expressed in M09CL and M35CL. The target genes of the differentially expressed miRNAs were significantly enriched in several biological processes, and the majority of these genes were involved in pathways in cancer and the MAPK signaling pathway. Through interaction analysis, 4 up-regulated miRNAs (cfa-miR-452, cfa-miR-8826, cfa-miR-224, and cfa-miR-2387) and their crucial target genes related to the tumor regulation network were identified. Results indicated these 4 miRNAs might play crucial roles in the tumorigenesis of MDCK cells.

conclusionOur findings, which were based on the functional prediction of miRNAs and target genes, suggested that miRNAs might influence the tumorigenicity of MDCK cells by regulating target genes. Moreover, the results provided important data for understanding the miRNA-mediated regulatory networks that control the tumorigenicities of MDCK cells.

Indexed as

MicroRNAsAnimalsCarcinogenesisDogsKidneyMadin Darby Canine Kidney CellsSignal TransductionMicroRNAsMDCK cellsmiRNA expressionSequence captureTumorigenicity

Identifiers

PMID35048333
OpenAlexW4206468038

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.