ArticleProceedings of the National Academy of Sciences of the United States of America2022
Probing molecular crowding in compressed tissues with Brillouin light scattering.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 24 citations in OpenAlex.
- Chromatin architecture and physical constriction cooperate in phenotype switching and cancer cell dissemination.bioRxiv : the preprint server for biology · 2026Article
- Stabilized real-time Brillouin microscopy reveals fractal organization of protein condensates in living cells.Nature communications · 2026Article
- Brillouin Microscopy of Breast tumor Spheroids On-a-Chip: Mechanical and Transcriptional Responses to Microfluidic-Induced Rapid Deformations.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Beyond Water Content: Unraveling Stiffness in Hydrated Materials by a Correlative Brillouin-Raman Approach.ACS photonics · 2025Article
- Mechanical Evolution of Metastatic Cancer Cells in 3D Microenvironment.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- The viscoelastic properties of Nicotiana tabacum BY-2 suspension cell lines adapted to high osmolarity.BMC plant biology · 2025Article
- Investigation of corneal hydration and the impact of cross-linking therapy on water retention using Brillouin spectroscopy, Raman spectroscopy and polarization-sensitive optical coherence tomography.Frontiers in bioengineering and biotechnology · 2025Article
- Article
- Mechanical evolution of metastatic cancer cells in three-dimensional microenvironment.bioRxiv : the preprint server for biology · 2024Article
- Estimation of the mass density of biological matter from refractive index measurements.Biophysical reports · 2024Article
- Probing molecular crowding in compressed tissues with Brillouin light scattering.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Volume regulation is key in maintaining important tissue functions, such as growth or healing. This is achieved by modulation of active contractility as well as water efflux that changes molecular crowding within individual cells. Local sensors have been developed to monitor stresses or forces in model tissues, but these approaches do not capture the contribution of liquid flows to volume regulation. Here, we use a tool based on Brillouin light scattering (BLS) that uses the interaction of a laser light with inherent picosecond timescale density fluctuations in the sample. To investigate volume variations, we induced osmotic perturbations with a polysaccharide osmolyte, Dextran (Dx), and compress cells locally within multicellular spheroids (MCSs). During osmotic compressions, we observe an increase in the BLS frequency shift that reflects local variations in the compressibility. To elucidate these data, we propose a model based on a mixing law that describes the increase of molecular crowding upon reduction of the intracellular fluids. Comparison with the data suggests a nonlinear increase of the compressibility due to the dense crowding that induces hydrodynamic interactions between the cellular polymers.
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