ArticleBMC plant biology2022
Nitrogen application and differences in leaf number retained after topping affect the tobacco (Nicotiana tabacum) transcriptome and metabolome.
Article in BMC plant biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 18 citations in OpenAlex.
- Combined transcriptome and metabolome analyses reveal that galactose metabolism is vital for tobacco in response to low-nitrate stress.Journal of genetics · 2026Article
- Comprehensive effects of functional agents on growth, nutrient accumulation, and rhizosphere bacterial communities in flue-cured tobacco (Frontiers in plant science · 2026Article
- Multi-omics analyses reveal regulatory networks underpinning metabolite biosynthesis in Nicotiana tabacum.Nature communications · 2025Article
- WGCNA analysis and identification of key genes in tobacco in response to different nitrogen levels.BMC plant biology · 2025Article
- Topping influences crop photosynthesis and alters the absorption and redistribution of nutrients: a case study of tobacco.Frontiers in plant science · 2025Article
- Proteomic and metabolomic insights into the impact of topping treatment on cigar tobacco.Frontiers in plant science · 2024Article
- Transcriptome Profiling and Chlorophyll Metabolic Pathway Analysis Reveal the Response ofPlants (Basel, Switzerland) · 2023Article
- Spatial Genomic Resource Reveals Molecular Insights into Key Bioactive-Metabolite Biosynthesis in EndangeredInternational journal of molecular sciences · 2022Article
- Uncovering Pathways Highly Correlated to NUE through a Combined Metabolomics and Transcriptomics Approach in Eggplant.Plants (Basel, Switzerland) · 2022Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAgronomic treatments such as the application of nitrogen fertilizer and topping (removal of the inflorescence and top leaves) cause substantial changes in plant metabolism. To explore these changes, we conducted comparative transcriptomic and metabolomic analyses of leaves collected from four positions along the stem on plants exposed to two nitrogen doses and with different numbers of leaves retained after topping in tobacco (Nicotiana tabacum).
resultsWe identified 13,330 unique differentially expressed genes and 32 differentially abundant metabolites. Through RNA-seq and WGCNA analyze, we constructed 2 co-expression networks (green and blue) highly correlation to N application and leaf number retained, predicted a hub gene NtGER3 may play an important role in N metabolism related to amino acid (cysteine) through CK pathway in tobacco leaves, NtARFs may participated in modulating the auxin signal and N in bottom leaves and NtRAP2.12 as key gene involved in N regulation by ethylene pathway. What's more, our data prove C/N transformation and balance affect the "source - flow - sink" redistribution and remobilization in tobacco during growth and development process.
conclusionsOverall, this comparative transcriptomics study provides novel insight into the complex molecular mechanisms underlying plant responses to different levels of nitrogen application and the number of leaves remaining after topping in plants.
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