Evidence map›Paper›PMID 35044725›Full record

ArticleCurrent protocols2022

Application of AAV1 for Anterograde Transsynaptic Circuit Mapping and Input-Dependent Neuronal Cataloging.

Brian Zingg, Hong-Wei Dong, Huizhong Whit Tao, Li I Zhang

Abstract read
In one paragraph

Article in Current protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Rostral Ventromedial Medulla (RVM) Projects to the Lateral Hypothalamic Area (LHA) to Drive Aversion and Anxiety.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Article
  12. Immunoception: the insular cortex perspective.Cellular & molecular immunology · 2023
    Review
  13. Article
  14. Article
  15. Review
  16. Review
  17. Review
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Brian ZinggDepartment of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California.
Hong-Wei DongDepartment of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California.
Huizhong Whit TaoZilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, Los Angeles, California.
Li I ZhangZilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, Los Angeles, California.

Funding

Cell atlas of mouse brain-spinal cord connectomeU01MH116990 · NIMH · UNIVERSITY OF SOUTHERN CALIFORNIA · PI DONG, HONG-WEI, TAO, HUIZHONG WHIT · 2018 to 2022
$12.0M
Processing and transformation of visual signals in the superior colliculusR01EY019049 · NEI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI TAO, HUIZHONG WHIT · 2008 to 2025
$9.2M
Thalamic Modulation of Auditory Cortical Processing via LPR01DC008983 · NIDCD · UNIVERSITY OF SOUTHERN CALIFORNIA · PI Li I Zhang · 2007 to 2026
$8.7M
Integrative approach to classifying neuronal cell types of the mouse hippocampusRF1MH114112 · NIMH · UNIVERSITY OF SOUTHERN CALIFORNIA · PI DONG, HONG-WEI, ZHANG, LI I · 2017 to 2017
$4.2M
NEI NIH HHS R01 EY019049NIDCD NIH HHS R01 DC008983NIH HHS R01DC008983NIH HHS R01EY019049NIH HHS U01MH116990NIMH NIH HHS RF1 MH114112NIMH NIH HHS U01 MH116990
6 · The paper itself

Abstract

Viruses that spread transsynaptically provide a powerful means to study interconnected circuits in the brain. Here we describe the use of adeno-associated virus, serotype 1 (AAV1), as a tool to achieve robust, anterograde transsynaptic spread in a variety of unidirectional pathways. A protocol for performing intracranial AAV1 injections in mice is presented, along with additional guidance for planning experiments, sourcing materials, and optimizing the approach to achieve the most successful outcomes. By following the methods presented here, researchers will be able to reveal postsynaptically connected neurons downstream of a given AAV1 injection site and access these input-defined cells for subsequent mapping, recording, and manipulation to characterize their anatomical and functional properties. © 2022 Wiley Periodicals LLC. Basic Protocol: Stereotaxic injection of AAV1 for anterograde transsynaptic spread.

Indexed as

DependovirusNeuronsAnimalsBrainMiceNeural PathwaysSerogroupAAVanterograde transsynaptic virusviral tracing

Identifiers

PMID35044725
PMCPMC8852298

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.