Evidence map›Paper›PMID 35044219›Full record

ArticleMicrobiology spectrum2022

Intracellular Transposition and Capture of Mobile Genetic Elements following Intercellular Conjugation of Multidrug Resistance Conjugative Plasmids from Clinical

Supathep Tansirichaiya, Richard N Goodman, Xinyu Guo, Issra Bulgasim, Ørjan Samuelsen, Mohammed Al-Haroni, Adam P Roberts

Open access · goldAbstract read
In one paragraph

Article in Microbiology spectrum, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.0field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
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  6. Molecular characterization and antibiotic resistance ofFood science and biotechnology · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 3 countries.

Supathep TansirichaiyaDepartment of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.ORCID 0000-0002-4445-8529
Richard N GoodmanDepartment of Tropical Disease Biology, Liverpool School of Tropical Medicinegrid.48004.38, Liverpool, United Kingdom.
Xinyu GuoDepartment of Tropical Disease Biology, Liverpool School of Tropical Medicinegrid.48004.38, Liverpool, United Kingdom.
Issra BulgasimDepartment of Tropical Disease Biology, Liverpool School of Tropical Medicinegrid.48004.38, Liverpool, United Kingdom.
Ørjan SamuelsenDepartment of Pharmacy, UiT the Arctic University of Norway, Tromsø, Norway.ORCID 0000-0002-5525-2614
Mohammed Al-HaroniDepartment of Clinical Dentistry, Faculty of Health Sciences, UiT the Arctic University of Norway, Tromsø, Norway.
Adam P RobertsDepartment of Tropical Disease Biology, Liverpool School of Tropical Medicinegrid.48004.38, Liverpool, United Kingdom.ORCID 0000-0002-0760-3088
Liverpool School of Tropical Medicine · GBSiriraj Hospital · THUiT The Arctic University of Norway · NOUniversity Hospital of North Norway · NO

Funding

Department of Health NIHR200632Medical Research Council MR/N013514/1Medical Research Council MR/S004793/1
6 · The paper itself

Abstract

Mobile genetic elements (MGEs) are often associated with antimicrobial resistance genes (ARGs). They are responsible for intracellular transposition between different replicons and intercellular conjugation and are therefore important agents of ARG dissemination. Detection and characterization of functional MGEs, especially in clinical isolates, would increase our understanding of the underlying pathways of transposition and recombination and allow us to determine interventional strategies to interrupt this process. Entrapment vectors can be used to capture active MGEs, as they contain a positive selection genetic system conferring a selectable phenotype upon the insertion of an MGE within certain regions of that system. Previously, we developed the pBACpAK entrapment vector that results in a tetracycline-resistant phenotype when MGEs translocate and disrupt the

Indexed as

Conjugation, GeneticDNA Transposable ElementsDrug Resistance, Multiple, BacterialAnti-Bacterial AgentsEnterobacteriaceaeEnterobacteriaceae InfectionsEscherichia coliGene Transfer, HorizontalHumansKlebsiella pneumoniaePlasmidsAnti-Bacterial AgentsDNA Transposable Elementsantimicrobial resistanceblaNDM-1entrapment vectorinsertion sequencetransposon

Identifiers

PMID35044219
PMCPMC8768599
OpenAlexW4206800375

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.