ArticleScientific reports2022
Identification of a fall armyworm (Spodoptera frugiperda)-specific gene and development of a rapid and sensitive loop-mediated isothermal amplification assay.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Recombinase polymerase amplification assay for sensitive and rapid detection of invasive fall armyworm, Spodoptera frugiperda.Scientific reports · 2025Article
- Rapid detection of the invasive tomato leaf miner, Phthorimaea absoluta using simple template LAMP assay.Scientific reports · 2025Article
- Major biotic stresses affecting maize production in Kenya and their implications for food security.PeerJ · 2023Review
- Development of a Rapid, Accurate, and On-Site Detection Protocol for Red Imported Fire Ants,Bioengineering (Basel, Switzerland) · 2022Article
- Fall armyworm from a maize multi-peril pest risk perspective.Frontiers in insect science · 2022Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The fall armyworm [FAW, Spodoptera frugiperda (J E Smith)], a moth native to America, has spread throughout the world since it was first discovered in Africa in 2016. The FAW is a polyphagous migratory pest that can travel over long distances using seasonal winds or typhoons because of its excellent flying ability, causing serious damage to many crops. For effective FAW control, accurate species identification is essential at the beginning of the invasion. In this study, the FAW-specific gene Sf00067 was discovered by performing bioinformatics to develop a fast and accurate tool for the species-specific diagnosis of this pest. An Sf00067 loop-mediated isothermal amplification (LAMP) assay was developed, and optimal conditions were established. The Sf00067 6 primer LAMP (Sf6p-LAMP) assay established in this study was able to diagnose various genotype-based strains of FAW captured in Korea and FAWs collected from Benin, Africa. Our FAW diagnostic protocol can be completed within 30 min, from the process of extracting genomic DNA from an egg or a 1st instar larva to species determination.
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