Evidence map›Paper›PMID 35042817›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2022

Multiscale X-ray study of

David N Azulay, Oliver Spaeker, Mnar Ghrayeb, Michaela Wilsch-Bräuninger, Ernesto Scoppola, Manfred Burghammer, Ivo Zizak, Luca Bertinetti, Yael Politi, Liraz Chai

Abstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
4.6field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 57 citations in OpenAlex.

  1. Article
  2. Article
  3. Extracellular matrix chemistry tunes bacterial biofilm metabolism and optimizes fitness.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  4. Article
  5. Article
  6. Mechanical Comparison ofACS biomaterials science & engineering · 2025
    Article
  7. Article
  8. Article
  9. Review
  10. Review
  11. Article
  12. Article
  13. Article
  14. How bacteria actively use passive physics to make biofilms.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  15. Where bacteria and eukaryotes meet.Journal of bacteriology · 2024
    Article
  16. Bacteriophage-mediated approaches for biofilm control.Frontiers in cellular and infection microbiology · 2024
    Review
  17. Article
  18. Review
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 6 institutions in 3 countries.

David N AzulayInstitute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.ORCID 0000-0002-6983-0446
Oliver SpaekerDepartment of Biomaterials, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany.ORCID 0000-0002-4542-120X
Mnar GhrayebInstitute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Michaela Wilsch-BräuningerMax Planck Institute of Molecular Cell Biology and Genetics, 1307 Dresden, Germany.ORCID 0000-0002-7217-6879
Ernesto ScoppolaDepartment of Biomaterials, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany.ORCID 0000-0002-6390-052X
Manfred BurghammerEuropean Synchrotron Radiation Facility, Grenoble 38043, France.ORCID 0000-0002-2262-3431
Ivo ZizakDepartment Structure and Dynamics of Energy Materials, Helmholtz-Zentrum Berlin, 12489 Berlin, Germany.
Luca BertinettiB CUBE Center for Molecular Bioengineering, Technische Universität Dresden, 01309 Dresden, Germany.
Yael PolitiB CUBE Center for Molecular Bioengineering, Technische Universität Dresden, 01309 Dresden, Germany liraz.chai@mail.huji.ac.il yael.politi@tu-dresden.de.ORCID 0000-0002-2872-8277
Liraz ChaiInstitute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel; liraz.chai@mail.huji.ac.il yael.politi@tu-dresden.de.ORCID 0000-0001-9644-4455
Hebrew University of Jerusalem · ILMax Planck Institute of Colloids and Interfaces · DETechnische Universität Dresden · DEEuropean Synchrotron Radiation Facility · FRHelmholtz-Zentrum Berlin für Materialien und Energie · DEMax Planck Institute of Molecular Cell Biology and Genetics · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Biofilms are multicellular microbial communities that encase themselves in an extracellular matrix (ECM) of secreted biopolymers and attach to surfaces and interfaces. Bacterial biofilms are detrimental in hospital and industrial settings, but they can be beneficial, for example, in agricultural as well as in food technology contexts. An essential property of biofilms that grants them with increased survival relative to planktonic cells is phenotypic heterogeneity, the division of the biofilm population into functionally distinct subgroups of cells. Phenotypic heterogeneity in biofilms can be traced to the cellular level; however, the molecular structures and elemental distribution across whole biofilms, as well as possible linkages between them, remain unexplored. Mapping X-ray diffraction across intact biofilms in time and space, we revealed the dominant structural features in

Indexed as

Amyloidogenic ProteinsBacillus subtilisBacterial ProteinsBiofilmsExtracellular MatrixIonsScattering, RadiationScattering, Small AngleX-RaysAmyloidogenic ProteinsBacterial ProteinsIonsbiofilmsextracellular matrixfunctional amyloid proteinsphenotypic heterogeneitysmall angle X-ray scattering (SAXS)/wide angle X-ray scattering (WAXS)

Identifiers

PMID35042817
PMCPMC8794879
OpenAlexW4220960797

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.