Evidence map›Paper›PMID 35041392›Full record

ArticleACS applied materials & interfaces2022

Specific Silencing of Microglial Gene Expression in the Rat Brain by Nanoparticle-Based Small Interfering RNA Delivery.

Shanshan Guo, Fernando Cázarez-Márquez, Han Jiao, Ewout Foppen, Nikita L Korpel, Anita E Grootemaat, Nalan Liv, Yuanqing Gao, Nicole van der Wel, Bing Zhou and 2 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS applied materials & interfaces, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 31 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 6 institutions in 2 countries.

Shanshan GuoDepartment of Endocrinology and Metabolism, Laboratory of Endocrinology, Amsterdam Gastroenterology Endocrinology Metabolism Research Institute, Amsterdam University Medical Centre (UMC), location AMC, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands.ORCID https://orcid.org/0000-0002-3880-4149
Fernando Cázarez-MárquezDepartment of Endocrinology and Metabolism, Laboratory of Endocrinology, Amsterdam Gastroenterology Endocrinology Metabolism Research Institute, Amsterdam University Medical Centre (UMC), location AMC, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands.
Han JiaoDepartment of Endocrinology and Metabolism, Laboratory of Endocrinology, Amsterdam Gastroenterology Endocrinology Metabolism Research Institute, Amsterdam University Medical Centre (UMC), location AMC, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands.
Ewout FoppenDepartment of Endocrinology and Metabolism, Laboratory of Endocrinology, Amsterdam Gastroenterology Endocrinology Metabolism Research Institute, Amsterdam University Medical Centre (UMC), location AMC, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands.
Nikita L KorpelDepartment of Endocrinology and Metabolism, Laboratory of Endocrinology, Amsterdam Gastroenterology Endocrinology Metabolism Research Institute, Amsterdam University Medical Centre (UMC), location AMC, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands.
Anita E GrootemaatCellular Imaging Core Facility, Amsterdam University Medical Centre (UMC), location AMC, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands.
Nalan LivSection Cell Biology, Center for Molecular Medicine, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands.
Yuanqing GaoKey Laboratory of Cardiovascular and Cerebrovascular Medicine, School of Pharmacy, Nanjing Medical University, Nanjing 211166, China.
Nicole van der WelCellular Imaging Core Facility, Amsterdam University Medical Centre (UMC), location AMC, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands.
Bing ZhouInstitute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Guangjun NieCAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing 100190, China.ORCID https://orcid.org/0000-0001-5040-9793
Chun-Xia YiDepartment of Endocrinology and Metabolism, Laboratory of Endocrinology, Amsterdam Gastroenterology Endocrinology Metabolism Research Institute, Amsterdam University Medical Centre (UMC), location AMC, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands.ORCID https://orcid.org/0000-0003-1184-4615
University of Amsterdam · NLNetherlands Institute for Neuroscience · NLNanjing Medical University · CNChinese Academy of Sciences · CNNational Center for Nanoscience and Technology · CNUniversity Medical Center Utrecht · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microglia are the major innate immune cells in the brain and are essential for maintaining homeostasis in a neuronal microenvironment. Currently, a genetic tool to modify microglial gene expression in specific brain regions is not available. In this report, we introduce a tailor-designed method that uses lipid and polymer hybridized nanoparticles (LPNPs) for the local delivery of small interfering RNAs (siRNAs), allowing the silencing of specific microglial genes in the hypothalamus. Our physical characterization proved that this LPNP-siRNA was uniform and stable. We demonstrated that, due to their natural phagocytic behavior, microglial cells are the dominant cell type taking up these LPNPs in the hypothalamus of rats. We then tested the silencing efficiency of LPNPs carrying a cluster of differentiation molecule 11b (CD11b) or Toll-like receptor 4 (TLR4) siRNA using different

Indexed as

AnimalsCD11b AntigenDrug CarriersGene ExpressionHypothalamusLipidsMaleMicrogliaNanoparticlesPolyestersPolyethylene GlycolsRatsRats, WistarRNA, Small InterferingToll-Like Receptor 4CD11b AntigenDrug CarriersLipidsmethoxypolyethyleneglycol-poly(lactic-co-glycolic acid)PolyestersPolyethylene GlycolsRNA, Small InterferingTlr4 protein, ratToll-Like Receptor 4CD11bhypothalamusmicrogliananoparticlesphagocytosissiRNATLR4

Identifiers

PMID35041392
PMCPMC8815040
OpenAlexW4206797543

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.