ArticleScientific reports2022
Low-invasive 5D visualization of mitotic progression by two-photon excitation spinning-disk confocal microscopy.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Fast Tissue Clearing and Volume Imaging Techniques for Anatomy.Microscopy research and technique · 2025Review
- Amyloid-β-induced alteration of fast and localized calcium elevations in cultured astrocytes.Scientific reports · 2025Article
- Cell Division Imaging of Tobacco BY-2 Cells in a 3D Volume by Two-Photon Spinning-Disk Confocal Microscopy.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Enucleation of theLife science alliance · 2024Article
- Improving two-photon excitation microscopy for sharper and faster biological imaging.Biophysics and physicobiology · 2023Article
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Authors and funding
8 authors.
Funding
Abstract
Non-linear microscopy, such as multi-photon excitation microscopy, offers spatial localities of excitations, thereby achieving 3D cross-sectional imaging with low phototoxicity even in thick biological specimens. We had developed a multi-point scanning two-photon excitation microscopy system using a spinning-disk confocal scanning unit. However, its severe color cross-talk has precluded multi-color simultaneous imaging. Therefore, in this study, we introduced a mechanical switching system to select either of two NIR laser light pulses and an image-splitting detection system for 3- or 4-color imaging. As a proof of concept, we performed multi-color fluorescent imaging of actively dividing human HeLa cells and tobacco BY-2 cells. We found that the proposed microscopy system enabled time-lapse multi-color 3D imaging of cell divisions while avoiding photodamage. Moreover, the application of a linear unmixing method to the 5D dataset enabled the precise separation of individual intracellular components in multi-color images. We thus demonstrated the versatility of our new microscopy system in capturing the dynamic processes of cellular components that could have multitudes of application.
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