Evidence map›Paper›PMID 35039068›Full record

ArticleBMC veterinary research2022

Occurrence of 'Candidatus Mycoplasma haemosuis' in fattening pigs, sows and piglets in Germany using a novel gap-based quantitative real-time PCR assay.

Julia Ade, Julia Stadler, Mathias Ritzmann, Christina Zübert, Katharina Hoelzle, Ludwig E Hoelzle

Open access · goldAbstract read
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Article in BMC veterinary research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.1field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Julia AdeInstitute of Animal Science, Department of Livestock Infectiology and Environmental Hygiene, University of Hohenheim, Garbenstr. 30, 70599, Stuttgart, Germany.
Julia StadlerClinic for Swine, Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-Universität Munich, Sonnenstrasse 16, 85764, Oberschleissheim, Germany.
Mathias RitzmannClinic for Swine, Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-Universität Munich, Sonnenstrasse 16, 85764, Oberschleissheim, Germany.
Christina ZübertInstitute of Animal Science, Department of Livestock Infectiology and Environmental Hygiene, University of Hohenheim, Garbenstr. 30, 70599, Stuttgart, Germany.
Katharina HoelzleInstitute of Animal Science, Department of Livestock Infectiology and Environmental Hygiene, University of Hohenheim, Garbenstr. 30, 70599, Stuttgart, Germany.
Ludwig E HoelzleInstitute of Animal Science, Department of Livestock Infectiology and Environmental Hygiene, University of Hohenheim, Garbenstr. 30, 70599, Stuttgart, Germany. ludwig.hoelzle@uni-hohenheim.de.
University of Hohenheim · DELudwig-Maximilians-Universität München · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe appearance of the novel porcine haemotrophic mycoplasma (HM) species 'Candidatus Mycoplasma haemosuis' was reported in apparently healthy but also in clinically sick animals in China, Korea and in a case report from Germany. Outside of Asia, however, nothing further is known about the frequency of 'Ca. M. haemosuis' in pigs to date. To investigate the distribution of this novel HM species in Germany, fattening pigs, sows and pre-suckling piglets were examined using a herein developed quantitative real-time PCR assay (qPCR). Because the piglets were sampled before the first colostrum uptake, additional information on a possible vertical transmission from dams to their offspring was obtained.

resultsOur novel qPCR assay successfully detected 'Ca. M. haemosuis' in all blood samples from the 'Ca. M. haemosuis'-infected pigs. No cross-reactivity was detected when DNA from non-target Mycoplasma spp. and other bacterial species representing 10

conclusion'Candidatus M. haemosuis' infection is present in German pig farms and the detection of the novel porcine HM species in piglets immediately after birth before colostrum intake indicates vertical transmission. The novel qPCR assay specific for 'Ca. M. haemosuis' described herein will be a prerequisite for future studies on the prevalence, epidemiology as well as the clinical and economic impact of 'Ca. M. haemosuis' infections.

Indexed as

MycoplasmaMycoplasma InfectionsSwine DiseasesAnimalsFemaleGermanyReal-Time Polymerase Chain ReactionSwine‘Candidatus Mycoplasma haemosuis’DiagnosisFarrowing sowsHaemotrophic mycoplasmasPre-suckling pigletsQuantitative PCRVertical transmission

Identifiers

PMID35039068
PMCPMC8762947
OpenAlexW4206211275

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.