ArticlePlant molecular biology2022
Root-specific CLE3 expression is required for WRKY33 activation in Arabidopsis shoots.
Article in Plant molecular biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- Single-Cell Transcriptomics Reveals FLS2-Dependent Hypoxia Signaling and ERF13-Mediated Transcription During flg22-Triggered Immunity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Peptide Hormone-Mediated Regulation of Plant Development and Environmental Adaptability.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Tri-arabinosylation facilitates the bioactivity of CLE3 peptide inPlant biotechnology (Tokyo, Japan) · 2025Article
- CaEMBO reports · 2025Article
- Research Progress of Small Plant Peptides on the Regulation of Plant Growth, Development, and Abiotic Stress.International journal of molecular sciences · 2024Review
- A cell-wall-modifying gene-dependent CLE26 peptide signaling confers drought resistance inPNAS nexus · 2024Article
- CLE peptide signaling in plant-microbe interactions.Frontiers in plant science · 2024Review
- Specificity models in MAPK cascade signaling.FEBS open bio · 2023Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
key messageThis study focused on the role of CLE1-7 peptides as defense mediators, and showed that root-expressed CLE3 functions as a systemic signal to regulate defense-related gene expression in shoots. In the natural environment, plants employ diverse signaling molecules including peptides to defend themselves against various pathogen attacks. In this study, we investigated whether CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE) genes (CLE1-7) respond to biotic stimuli. CLE3 showed significant up-regulation upon treatment with flg22, Pep2, and salicylic acid (SA). Quantitative real-time PCR (qRT-PCR) analysis revealed that CLE3 expression is regulated by the NON-EXPRESSOR OF PR GENES1 (NPR1)-dependent SA signaling and flg22-FLAGELLIN-SENSITIVE 2 (FLS2) signaling pathways. We demonstrated that SA-induced up-regulation of CLE3 in roots was required for activation of WRKY33, a gene involved in the regulation of systemic acquired resistance (SAR), in shoots, suggesting that CLE3 functions as a root-derived signal that regulates the expression of defense-related genes in shoots. Microarray analysis of transgenic Arabidopsis lines overexpressing CLE3 under the control of a β-estradiol-inducible promoter revealed that root-confined CLE3 overexpression affected gene expression in both roots and shoots. Comparison of CLE2- and CLE3-induced genes indicated that CLE2 and CLE3 peptides target a few common but largely distinct downstream genes. These results suggest that root-derived CLE3 is involved in the regulation of systemic rather than local immune responses. Our study also sheds light on the potential role of CLE peptides in long-distance regulation of plant immunity.
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