ArticleBiology methods & protocols2022
Desktop scanning electron microscopy in plant-insect interactions research: a fast and effective way to capture electron micrographs with minimal sample preparation.
Article in Biology methods & protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 14 citations in OpenAlex.
- Comparative evaluation of physical methods for extracting leaf trichomes.Plant methods · 2026Article
- Light and Electron Microscope Observations of the Male Reproductive Organs of Adult Julodis ehrenbergii Laporte (Coleoptera, Buprestidae).Microscopy research and technique · 2025Article
- Morphological characterization of intraspecific variation for trichome traits in tomato (Solanum lycopersicum).Botanical studies · 2023Article
- Cascading Effects of Cover Crops on the Subsequent Cash Crop Defense against the Polyphagous Herbivore Fall Armyworm (Insects · 2023Article
- Trichomes mediate plant-herbivore interactions in two Cucurbitaceae species through pre- and post-ingestive ways.Journal of pest science · 2023Article
- An attempt at fixing the oversimplification of Nightshades' (genusFrontiers in plant science · 2023Article
- Morphological characterization of trichomes shows enormous variation in shape, density and dimensions across the leaves of 14AoB PLANTS · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to visualize cell and tissue morphology at a high magnification using scanning electron microscopy (SEM) has revolutionized plant sciences research. In plant-insect interactions studies, SEM-based imaging has been of immense assistance to understand plant surface morphology including trichomes [plant hairs; physical defense structures against herbivores], spines, waxes, and insect morphological characteristics such as mouth parts, antennae, and legs, that they interact with. While SEM provides finer details of samples, and the imaging process is simpler now with advanced image acquisition and processing, sample preparation methodology has lagged. The need to undergo elaborate sample preparation with cryogenic freezing, multiple alcohol washes, and sputter coating makes SEM imaging expensive, time consuming, and warrants skilled professionals, making it inaccessible to majority of scientists. Here, using a desktop version of SEM (SNE- 4500 Plus Tabletop), we show that the "plug and play" method can efficiently produce SEM images with sufficient details for most morphological studies in plant-insect interactions. We used leaf trichomes of
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Registered trials
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