Article3 Biotech2022
Achieving maximum efficiency of
Article in 3 Biotech, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 12 citations in OpenAlex.
- Spot Inoculation of Begomovirus by Single Whitefly (Bemisia tabaci) Transmission in Mungbean Using Microcage.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Plant Biotic Stress: Tools and Techniques for Crop Protection.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A review of factors affecting the success of geminivirus infectious clones.Plant cell reports · 2025Review
- Assessment of soybean breeding lines from the all India coordinated research programme for agronomic traits and resistance to mungbean yellow mosaic India virus through field screening and artificial inoculation.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- Development of a TaqMan qPCR for the Simultaneous Detection of the TuMV and BBWV2 Viruses Responsible for the Viral Disease inMicroorganisms · 2024Article
- Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mungbean is one of the important food legumes in the Indian-sub-continent. Yellow mosaic disease, caused by Mungbean yellow mosaic virus and Mungbean yellow mosaic India virus (MYMIV) poses a severe threat to its production. Agroinoculation has been the most preferred way to test the function of genomic components of these viruses. However, the available inoculation methods are not as efficient as whitefly transmission, thereby limiting their usage for screening and biological studies. We hereby report an efficient and reproducible agroinoculation method for achieving maximum (100%) efficiency using tandem repeat infectious agro-constructs of DNA A and DNA B of MYMIV. The present study targeted wounding of various meristematic tissues of root, shoot, parts of germinating seeds and also non-meristematic tissue of stem to test the suitable tissue types for maximum infection. Among the various tissues selected for, the inoculation on the epicotyl region showed maximum infectivity. Further, to enhance the infectivity of MYMIV, different concentrations of acetosyringone, incubation time and SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13205-021-03088-w.
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Registered trials
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