Evidence map›Paper›PMID 35035141›Full record

ArticlePhysiology and molecular biology of plants : an international journal of functional plant biology2021

Identification and validation of reference genes for qRT-PCR based studies in horse gram (

Ragini Sinha, Meenu Bala, Pragya Prabha, Alok Ranjan, Rakesh K Chahota, Tilak Raj Sharma, Anil Kumar Singh

Open access · greenAbstract read
In one paragraph

Article in Physiology and molecular biology of plants : an international journal of functional plant biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Selection of Reference Genes inPlants (Basel, Switzerland) · 2024
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Ragini SinhaSchool of Genetic Engineering, ICAR-Indian Institute of Agricultural Biotechnology, Ranchi, 834010 India.
Meenu BalaSchool of Genetic Engineering, ICAR-Indian Institute of Agricultural Biotechnology, Ranchi, 834010 India.
Pragya PrabhaSchool of Genetic Engineering, ICAR-Indian Institute of Agricultural Biotechnology, Ranchi, 834010 India.
Alok RanjanSchool of Genetic Engineering, ICAR-Indian Institute of Agricultural Biotechnology, Ranchi, 834010 India.
Rakesh K ChahotaDepartment of Agricultural Biotechnology, Choudhary Sarwan Kumar Himachal Pradesh Agricultural University, Palampur, 176061 India.
Tilak Raj SharmaSchool of Genetic Engineering, ICAR-Indian Institute of Agricultural Biotechnology, Ranchi, 834010 India.
Anil Kumar SinghSchool of Genetic Engineering, ICAR-Indian Institute of Agricultural Biotechnology, Ranchi, 834010 India.ORCID 0000-0002-3569-5207
ICAR-Indian Institute of Agricultural Biotechnology · INChaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya · INNational Research Centre on Plant Biotechnology · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The quantitative real-time polymerase chain reaction (qRT-PCR) is the most sensitive and commonly used technique for gene expression studies in biological systems. However, the reliability of qRT-PCR results depends on the selection of reference gene(s) for data normalization. Horse gram ( SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12298-021-01104-0.

Indexed as

BestkeeperDelta-delta CTgeNormHousekeeping geneMacrotyloma uniflorumNormfinderRank aggregationRefFinder

Identifiers

PMID35035141
PMCPMC8720121
OpenAlexW3216062766

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.