ArticleMetabolic engineering2022
Harnessing plasmid replication mechanism to enable dynamic control of gene copy in bacteria.
Article in Metabolic engineering, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- A hybrid system enables plasmid copy number control in yeast.Nature communications · 2026Article
- A non‑natural shortcut biosynthetic route enables de novo microbial production of 3‑hydroxyanthranilic and quinolinic acids.World journal of microbiology & biotechnology · 2026Article
- Population-level amplification of gene regulation by programmable gene transfer.Nature chemical biology · 2025Article
- Recent advances in recombinant production of soluble proteins in E. coli.Microbial cell factories · 2025Review
- Seven critical challenges in synthetic one-carbon assimilation and their potential solutions.FEMS microbiology reviews · 2025Review
- Dynamic control of the plasmid copy number maintained without antibiotics in Escherichia coli.Journal of biological engineering · 2024Article
- Engineering plasmid copy number heterogeneity for dynamic microbial adaptation.Nature microbiology · 2024Article
- Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli.Archives of microbiology · 2024Review
- Engineered bacterial orthogonal DNA replication system for continuous evolution.Nature chemical biology · 2023Article
- Dynamic Metabolic Control: From the Perspective of Regulation Logic.Synthetic biology and engineering · 2023Article
- Identifying, Characterizing, and Engineering a Phenolic Acid-Responsive Transcriptional Factor fromACS synthetic biology · 2023Article
- Application of Metabolite-Responsive Biosensors for Plant Natural Products Biosynthesis.Biosensors · 2023Review
- Biosensor-assisted titratable CRISPRi high-throughput (BATCH) screening for over-production phenotypes.Metabolic engineering · 2023Article
- Inducible plasmid copy number control for synthetic biology in commonly used E. coli strains.Nature communications · 2022Article
- Establishing an Autonomous Cascaded Artificial Dynamic (AutoCAD) regulation system for improved pathway performance.Metabolic engineering · 2022Article
- Strategies for efficient production of recombinant proteins in Escherichia coli: alleviating the host burden and enhancing protein activity.Microbial cell factories · 2022Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Dynamic regulation has been proved efficient in controlling gene expression at transcriptional, translational, and post-translational level. However, the dynamic regulation at gene replication level has been rarely explored so far. In this study, we established dynamic regulation at gene copy level through engineering controllable plasmid replication to dynamically control the gene expression. Prototypic genetic circuits with different control logic were applied to enable diversified dynamic behaviors of gene copy. To explore the applicability of this strategy, the dynamic gene copy control was employed in regulating the biosynthesis of p-coumaric acid, which resulted in an up to 78% increase in p-coumaric acid titer to 1.69 g/L in shake flasks. These results indicated the great potential of applying dynamic gene copy control for engineering biosynthesis of valuable compounds in metabolic engineering.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.