Evidence map›Paper›PMID 35032693›Full record

ArticleCellular and molecular gastroenterology and hepatology2022

A Pro-Endocrine Pancreatic Islet Transcriptional Program Established During Development Is Retained in Human Gallbladder Epithelial Cells.

Mugdha V Joglekar, Subhshri Sahu, Wilson K M Wong, Sarang N Satoor, Charlotte X Dong, Ryan J Farr, Michael D Williams, Prapti Pandya, Gaurang Jhala, Sundy N Y Yang and 16 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular gastroenterology and hepatology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

26 authors at 8 institutions in 4 countries.

Mugdha V JoglekarDiabetes and Islet Biology Group, School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia.
Subhshri SahuDiabetes and Islet Biology Group, School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia.
Wilson K M WongDiabetes and Islet Biology Group, School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia.
Sarang N SatoorDiabetes and Islet Biology Group, School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia.
Charlotte X DongDiabetes and Islet Biology Group, School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia.
Ryan J FarrDiabetes and Islet Biology Group, School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia.
Michael D WilliamsDiabetes and Islet Biology Group, School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia.
Prapti PandyaDiabetes and Islet Biology Group, School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia.
Gaurang JhalaImmunology and Diabetes Group, St. Vincent's Institute for Medical Research, Victoria, Australia.
Sundy N Y YangDiabetes and Islet Biology Group, School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia.
Yi Vee ChewThe Westmead Institute for Medical Research, Westmead Millenium Institute, University of Sydney, Westmead, New South Wales, Australia.
Nicola HetheringtonDiabetes and Islet Biology Group, School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia.
Dhan ThiruchevlamDepartment of Gastroenterology, St. Vincent's Hospital, Melbourne, Victoria, Australia.
Sasikala MitnalaSurgical Gastroenterology Research, Asian Institute of Gastroenterology, Hyderabad, India.
Guduru V RaoSurgical Gastroenterology Research, Asian Institute of Gastroenterology, Hyderabad, India.
Duvvuru Nageshwar ReddySurgical Gastroenterology Research, Asian Institute of Gastroenterology, Hyderabad, India.
Thomas LoudovarisImmunology and Diabetes Group, St. Vincent's Institute for Medical Research, Victoria, Australia.
Wayne J HawthorneThe Westmead Institute for Medical Research, Westmead Millenium Institute, University of Sydney, Westmead, New South Wales, Australia.
Andrew G ElefantyMurdoch Childrens Research Institute, Parkville, Victoria, Australia.
Vinay M JoglekarShree Seva Medical Foundation, Shirwal, India.
Edouard G StanleyMurdoch Childrens Research Institute, Parkville, Victoria, Australia.
David MartinUpper Gastrointestinal Surgery, Strathfield Hospital, Strathfield, New South Wales, Australia.
Helen E ThomasImmunology and Diabetes Group, St. Vincent's Institute for Medical Research, Victoria, Australia.
David ToshDepartment of Biology and Biochemistry, University of Bath, Bath, United Kingdom.
Louise T DalgaardSection of Eukaryotic Cell Biology, Department of Science and Environment, Roskilde University, Roskilde, Denmark.
Anandwardhan A HardikarDiabetes and Islet Biology Group, School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia. Electronic address: a.hardikar@westernsydney.edu.au.
Camden and Campbelltown Hospitals · AUAsian Institute of Gastroenterology · INSt Vincents Institute of Medical Research · AUMurdoch Children's Research Institute · AUThe University of Sydney · AURoskilde University · DKSt Vincent's Hospital Melbourne · AUUniversity of Bath · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND &

aimsPancreatic islet β-cells are factories for insulin production; however, ectopic expression of insulin also is well recognized. The gallbladder is a next-door neighbor to the developing pancreas. Here, we wanted to understand if gallbladders contain functional insulin-producing cells.

methodsWe compared developing and adult mouse as well as human gallbladder epithelial cells and islets using immunohistochemistry, flow cytometry, enzyme-linked immunosorbent assays, RNA sequencing, real-time polymerase chain reaction, chromatin immunoprecipitation, and functional studies.

resultsWe show that the epithelial lining of developing, as well as adult, mouse and human gallbladders naturally contain interspersed cells that retain the capacity to actively transcribe, translate, package, and release insulin. We show that human gallbladders also contain functional insulin-secreting cells with the potential to naturally respond to glucose in vitro and in situ. Notably, in a non-obese diabetic (NOD) mouse model of type 1 diabetes, we observed that insulin-producing cells in the gallbladder are not targeted by autoimmune cells. Interestingly, in human gallbladders, insulin splice variants are absent, although insulin splice forms are observed in human islets.

conclusionsIn summary, our biochemical, transcriptomic, and functional data in mouse and human gallbladder epithelial cells collectively show the evolutionary and developmental similarities between gallbladder and the pancreas that allow gallbladder epithelial cells to continue insulin production in adult life. Understanding the mechanisms regulating insulin transcription and translation in gallbladder epithelial cells would help guide future studies in type 1 diabetes therapy.

Indexed as

Diabetes Mellitus, Type 1Islets of LangerhansAnimalsEpithelial CellsGallbladderHumansInsulinMiceMice, Inbred NODInsulinDifferentiationGallbladder DevelopmentInsulinIsletsSplice Variants

Identifiers

PMID35032693
PMCPMC9043310
OpenAlexW4205540819

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.