Evidence map›Paper›PMID 35031914›Full record

ArticleAntonie van Leeuwenhoek2022

Identification, characterization and hydrolase producing performance of thermophilic bacteria: geothermal hot springs in the Eastern and Southeastern Anatolia Regions of Turkey.

Orhan Ulucay, Arzu Gormez, Cem Ozic

Abstract read
In one paragraph

Article in Antonie van Leeuwenhoek, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Characterization of crude cellulase produced by Bacillus licheniformis OE-1 from the Diyadin hot springs, Ağrı Province.International microbiology : the official journal of the Spanish Society for Microbiology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Orhan UlucayDepartment of Bioengineering, Faculty of Engineering and Architecture, Kafkas University, 36100, Kars, Turkey. orhanulucay@kafkas.edu.tr.ORCID http://orcid.org/0000-0002-0820-5372
Arzu GormezDepartment of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, 25050, Yakutiye, Erzurum, Turkey.
Cem OzicDepartment of Medical Biology, Medicine Faculty, Kafkas University, 36100, Kars, Turkey.

Funding

Kafkas Üniversitesi BAP KAU 2016-FM-24
6 · The paper itself

Abstract

In the last two decades, researchers have increasingly focused on the rich microorganism-based diversity of natural hot spring sources to explore the benefits of thermophiles in industrial and biotechnological fields. Within the scope of this study, a total of 83 thermophilic Bacilli strains were isolated from 7 different geothermal hot springs (at temperatures ranging between 40 and 85 °C) located in the Eastern and Southeastern Anatolia Regions of Turkey. The physiological, morphological, biochemical and molecular properties of the isolates were determined. As a result of the 16S rRNA gene sequence analysis, 5 different species (Bacillus licheniformis, Bacillus sp., Bacillus subtilis, Geobacillus kaustophilus, and Weizmannia coagulans,) were identified. B. licheniformis and B. subtilis were the most frequently encountered species among those obtained from the researched hot spring sources. Phylogenetic analysis was conducted to evaluate the phylogenetic relationships of the isolated species. The results showed that there was no significant difference between the groups and the bacteria in terms of the locations or optimum temperatures of the isolates. The bacterial isolates were screened for amylase, cellulase, lipase and protease hydrolytic enzyme activities. The hydrolytic enzyme production potentials among the isolates were identified in 68 (82%) isolates for amylase, 34 (41%) for cellulase, 69 (83%) for lipase and 73 (88%) for protease. All isolates were found to have at least one or more extracellular enzyme activities. Additionally, it was determined that 27 of the existing isolates (32.8%) were able to produce all of the aforementioned hydrolytic enzymes.

Indexed as

Hot SpringsHot TemperatureLipasePhylogenyRNA, Ribosomal, 16STurkeyLipaseRNA, Ribosomal, 16SBacillus subtilisEnzyme activityGeobacillusHot springsThermophilic bacteria

Identifiers

PMID35031914
PMCPMC8760091

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.