ArticleHorticulture research2022
Alternative transcription and feedback regulation suggest that SlIDI1 is involved in tomato carotenoid synthesis in a complex way.
Article in Horticulture research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 22 citations in OpenAlex.
- Splicing regulation by RS2Z36 controls ovary patterning and fruit growth in tomato.Plant molecular biology · 2026Article
- Decoding Isoprenoid Transcript-Metabolite Interactions in Carotenoid Tomato Fruit Mutants Uncovers Novel Metabolic Cross-Links.International journal of molecular sciences · 2026Article
- Identification and functional characterization of a novel gene conferring root rot resistance in Panax Notoginseng.BMC plant biology · 2026Article
- QTL mapping for young leaf color trait in eggplant (Frontiers in genetics · 2026Article
- Heterologous Biosynthesis of Crocin I inInternational journal of molecular sciences · 2025Article
- Alternative Splicing of Functional Genes in Plant Growth, Development, and Stress Responses.International journal of molecular sciences · 2025Review
- Molecular breeding of tomato: Advances and challenges.Journal of integrative plant biology · 2025Review
- Mutation of YFT3, an isomerase in the isoprenoid biosynthetic pathway, impairs its catalytic activity and carotenoid accumulation in tomato fruit.Horticulture research · 2024Article
- A natural variation inHorticulture research · 2024Article
- 15-Foods (Basel, Switzerland) · 2024Article
- Integrative Metabolome and Transcriptome Analyses Provide Insights into Carotenoid Variation in Different-Colored Peppers.International journal of molecular sciences · 2023Article
- Current insights into posttranscriptional regulation of fleshy fruit ripening.Plant physiology · 2023Review
- CRISPR/Cas9-mediated SNAC9 mutants reveal the positive regulation of tomato ripening by SNAC9 and the mechanism of carotenoid metabolism regulation.Horticulture research · 2023Article
- Recoloring tomato fruit by CRISPR/Cas9-mediated multiplex gene editing.Horticulture research · 2023Article
- Mapping and Validation ofInternational journal of molecular sciences · 2022Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carotenoid pigments confer photoprotection and visual attraction and serve as precursors for many important signaling molecules. Herein, the orange-fruited phenotype of a tomato elite inbred line resulting from sharply reduced carotenoid levels and an increased β-carotene-to-lycopene ratio in fruit was shown to be controlled by a single recessive gene, oft3. BSA-Seq combined with fine mapping delimited the oft3 gene to a 71.23 kb interval on chromosome 4, including eight genes. Finally, the oft3 candidate gene SlIDI1, harboring a 116 bp deletion mutation, was identified by genome sequence analysis. Further functional complementation and CRISPR-Cas9 knockout experiments confirmed that SlIDI1 was the gene underlying the oft3 locus. qRT-PCR analysis revealed that the expression of SlIDI1 was highest in flowers and fruit and increased with fruit ripening or flower maturation. SlIDI1 simultaneously produced long and short transcripts by alternative transcription initiation and alternative splicing. Green fluorescent protein fusion expression revealed that the long isoform was mainly localized in plastids and that an N-terminal 59-amino acid extension sequence was responsible for plastid targeting. Short transcripts were identified in leaves and fruit by 5' RACE and in fruit by 3' RACE, which produced corresponding proteins lacking transit peptides and/or putative peroxisome targeting sequences, respectively. In SlIDI1 mutant fruit, SlBCH1 transcription involved in β-carotenoid catabolism was obviously suppressed, which may be responsible for the higher β-carotene-to-lycopene ratio and suggested potential feedback regulatory mechanisms involved in carotenoid pathway flux.
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