ArticlePloS one2022
Insights into cell wall disintegration of Chlorella vulgaris.
Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 124 citations in OpenAlex.
- Algae Ingestion Increases Resting and Exercised Myofibrillar Protein Synthesis Rates to a Similar Extent as Mycoprotein in Young Adults.The Journal of nutrition · 2023Trial
- Enzymatic hydrolysis of Chlorella sorokiniana biomass using fungal enzyme mixtures: screening, optimization, and proteomic analysis for enhanced microalgal biomass valorization.Bioprocess and biosystems engineering · 2026Article
- Microalgae-Derived Proteins for Sustainable Foods and Beverages: Sources, Extraction, Characterization, and Applications.Foods (Basel, Switzerland) · 2026Review
- From Pond to Plate: Tailored Functional Proteins for Next-Gen Microalgal Food.Foods (Basel, Switzerland) · 2026Review
- Extrusion and Pancreatin Superdosing Modulate the Metabolic Impact of 20% Chlorella vulgaris Inclusion in Broiler Diets.Journal of animal physiology and animal nutrition · 2026Article
- Biochemical and Antioxidant Characterization of Pigment-DeficientFoods (Basel, Switzerland) · 2026Article
- Bioaccessibility and bioavailability assessment of microalgae-derived minerals for human nutrition.Current research in food science · 2026Article
- Surface-dependent reorganization ofCell surface (Amsterdam, Netherlands) · 2025Article
- Neuroprotective effects of Chlorella vulgaris loaded niosomes via SIRT1 activation in aluminum chloride-induced Alzheimer's model.Scientific reports · 2025Article
- Acylpyruvates and Their Heterocyclic Derivatives as Growth Regulators inBiotech (Basel (Switzerland)) · 2025Article
- Toxicological Responses of Photosynthetic Genes inInternational journal of molecular sciences · 2025Article
- Ecotoxicological Assessment and Biodegradation Potential of Epoxidised Methyl Oleate (EMO) as a Bio-Based Plasticiser in Aquatic Environment.Tropical life sciences research · 2025Article
- Microalgal Bioethanol Production for Sustainable Development: Current Status and Future Prospects.Indian journal of microbiology · 2025Article
- Cell walls: a comparative view of the composition of cell surfaces of plants, algae, and microorganisms.Journal of experimental botany · 2025Review
- Oral Exposure toToxins · 2025Article
- Biphosphor Carbon Dots/Chlorophyll System Entirely Derived fromACS nanoscience Au · 2025Article
- Chlorella vulgaris mutants with altered cell walls show increased permeability and enhanced extractability of intracellular molecules.Biotechnology for biofuels and bioproducts · 2025Article
- Phylogenetic Analysis, Pulse-Amplitude-Modulated (PAM) Fluorometry Measuring Parameter Optimization, and Cell Wall Disintegration ofMicroorganisms · 2025Article
- Improving protein hydrolysis and digestibility inHeliyon · 2025Article
- Impact of Nanoplastics on the Functional Profile of Microalgae Species Used as Food Supplements: Insights from Comparative In Vitro and Ex Vivo Digestion Studies.Journal of agricultural and food chemistry · 2025Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With their ability of CO2 fixation using sunlight as an energy source, algae and especially microalgae are moving into the focus for the production of proteins and other valuable compounds. However, the valorization of algal biomass depends on the effective disruption of the recalcitrant microalgal cell wall. Especially cell walls of Chlorella species proved to be very robust. The wall structures that are responsible for this robustness have been studied less so far. Here, we evaluate different common methods to break up the algal cell wall effectively and measure the success by protein and carbohydrate release. Subsequently, we investigate algal cell wall features playing a role in the wall's recalcitrance towards disruption. Using different mechanical and chemical technologies, alkali catalyzed hydrolysis of the Chlorella vulgaris cells proved to be especially effective in solubilizing up to 56 wt% protein and 14 wt% carbohydrates of the total biomass. The stepwise degradation of C. vulgaris cell walls using a series of chemicals with increasingly strong conditions revealed that each fraction released different ratios of proteins and carbohydrates. A detailed analysis of the monosaccharide composition of the cell wall extracted in each step identified possible factors for the robustness of the cell wall. In particular, the presence of chitin or chitin-like polymers was indicated by glucosamine found in strong alkali extracts. The presence of highly ordered starch or cellulose was indicated by glucose detected in strong acidic extracts. Our results might help to tailor more specific efforts to disrupt Chlorella cell walls and help to valorize microalgae biomass.
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