Evidence map›Paper›PMID 35029749›Full record

ArticleClinical oral investigations2022

Periodontal ligament cells under mechanical force regulate local immune homeostasis by modulating Th17/Treg cell differentiation.

Jiayu Lin, Jiachang Huang, Zhaoqiang Zhang, Xinyi Yu, Xuepei Cai, Chufeng Liu

Abstract read
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In one paragraph

Article in Clinical oral investigations, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 17 citations in OpenAlex.

  1. [Research Progress of Peripheral Immune Tolerance in Oral Diseases].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Jiayu Lin *Department of Orthodontics, Stomatological Hospital, Southern Medical University, No.366, Jiangnan Avenue South, Guangzhou, 510280, China.
Jiachang Huang *Department of Orthodontics, Stomatological Hospital, Southern Medical University, No.366, Jiangnan Avenue South, Guangzhou, 510280, China.
Zhaoqiang Zhang *Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Southern Medical University, No.366, Jiangnan Avenue South, Guangzhou, 510280, China.
Xinyi YuDepartment of Orthodontics, Stomatological Hospital, Southern Medical University, No.366, Jiangnan Avenue South, Guangzhou, 510280, China.
Xuepei CaiDepartment of Orthodontics, Stomatological Hospital, Southern Medical University, No.366, Jiangnan Avenue South, Guangzhou, 510280, China.
Chufeng LiuDepartment of Orthodontics, Stomatological Hospital, Southern Medical University, No.366, Jiangnan Avenue South, Guangzhou, 510280, China. xyzmmok@smu.edu.cn.ORCID http://orcid.org/0000-0002-3995-0148
Southern Medical University · CNGuangdong Province Stomatological Hospital · CN

Funding

natural science foundation of guangdong province No. 2018A0303130261
6 · The paper itself

Abstract

objectivesImproper orthodontic force often causes root resorption or destructive bone resorption. There is evidence that T helper 17 (Th17) cells and regulatory T (Treg) cells may be actively involved in bone remodeling during tooth movement. In a combination of in vitro and in vivo studies, we investigated the effect of human periodontal ligament cells (hPDLCs) on Th17/Treg cells under different orthodontic forces and corticotomy. MATERIAL AND

methodshPDLCs were cultured in vitro and subjected to different mechanical forces. The expression of interleukin (IL)-6 and transforming growth factor (TGF)-β in the supernatant and the mRNA levels of hypoxia inducible factor (HIF)-1α, Notch1, and TGF-β in hPDLCs were investigated. Supernatants were collected and co-cultured with activated CD4

resultsHeavy force repressed cell viability and increased the mortality rate of hPDLCs; it also improved the expression of IL-6, declined the expression of TGF-β, and promoted the mRNA expression level of HIF-1α. The expression of TGF-β and Notch1 mRNA decreased and then increased. The supernatant of hPDLCs under heavy force promotes the polarization of Th17 cells. The heavy force caused root resorption and decreased alveolar bone height and increased the positive area of IL-17A immunohistochemical staining and the expression of IL-17A, IL-6, HIF-1α, and Notch1 mRNA. Corticotomy accelerated tooth movement, increased the proportion of Foxp3-positive cells, and up-regulated the expression of Foxp3, IL-10, and CXCL12 mRNA.

conclusionsDuring orthodontic tooth movement, the heavy force causes root resorption and inflammatory bone destruction, which could be associated with increased expression of Th17 cells and IL-6. Corticotomy can accelerate tooth movement without causing root resorption and periodontal bone loss, which may be related to the increased expression of Treg cells. CLINICAL RELEVANCE: Altogether, this report provides a new perspective on the prevention of inflammatory injury via the regulation of Th17/Treg cells in orthodontics.

Indexed as

Alveolar Bone LossT-Lymphocytes, RegulatoryAnimalsCell DifferentiationHomeostasisPeriodontal LigamentTh17 CellsTooth Movement TechniquesX-Ray MicrotomographyCorticotomyOrthodonticsPeriodontal ligament cellsTh17 cellsTreg cells

Identifiers

PMID35029749
OpenAlexW4206010640

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.