Evidence map›Paper›PMID 35029688›Full record

ArticleTransactions of the Royal Society of Tropical Medicine and Hygiene2022

Molecular characterisation of carbapenem-resistant Klebsiella pneumoniae clinical isolates: preliminary experience from a tertiary care teaching hospital in the Himalayas.

Mohit Bhatia, Varun Shamanna, Geetha Nagaraj, Dharmavaram Sravani, Pratima Gupta, Balram Ji Omar, Deepika Chakraborty, K L Ravikumar

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Article in Transactions of the Royal Society of Tropical Medicine and Hygiene, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1 · What the graph read from it

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Mohit BhatiaDepartment of Microbiology, All India Institute of Medical Sciences Rishikesh, Uttarakhand, India 249203.ORCID 0000-0003-2001-8702
Varun ShamannaCentral Research Laboratory, Kempegowda Institute of Medical Sciences, Bengaluru, Karnataka, India 560070.ORCID 0000-0003-2775-2280
Geetha NagarajCentral Research Laboratory, Kempegowda Institute of Medical Sciences, Bengaluru, Karnataka, India 560070.ORCID 0000-0001-8099-7493
Dharmavaram SravaniCentral Research Laboratory, Kempegowda Institute of Medical Sciences, Bengaluru, Karnataka, India 560070.ORCID 0000-0003-0594-4855
Pratima GuptaDepartment of Microbiology, All India Institute of Medical Sciences Rishikesh, Uttarakhand, India 249203.ORCID 0000-0002-2971-5355
Balram Ji OmarDepartment of Microbiology, All India Institute of Medical Sciences Rishikesh, Uttarakhand, India 249203.ORCID 0000-0001-7805-2730
Deepika ChakrabortyDepartment of Microbiology, All India Institute of Medical Sciences Rishikesh, Uttarakhand, India 249203.ORCID 0000-0003-3270-6457
K L RavikumarCentral Research Laboratory, Kempegowda Institute of Medical Sciences, Bengaluru, Karnataka, India 560070.ORCID 0000-0001-8629-1459

Funding

Department of Health 16_136_111Wellcome TrustWellcome Trust 206194
6 · The paper itself

Abstract

backgroundThere is a lack of whole-genome sequencing (WGS) data on multidrug-resistant (MDR) bacteria from the Uttarakhand region of India. The aim of this study was to generate WGS data of carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates recovered from patients in Uttarakhand's tertiary care centre.

methodsA cross-sectional study included 29 MDR K. pneumoniae test isolates obtained from various clinical samples submitted to the bacteriology laboratory for culture and sensitivity testing from July 2018 to August 2019. After preliminary identification and antibiotic susceptibility testing, these isolates were subjected to WGS.

resultsA total of 27 of 29 isolates were CRKP. ST14 was the most common sequence type (n=8 [29.6%]). Carbapenem resistance was mainly encoded by OXA-48-like genes (21/27 [77.8%]). All isolates had a varied arsenal of resistance genes to different antibiotic classes. KL2 (9/27 [33.3%]) and KL51 (8/27 [29.6%]) were dominant K loci types. O1 and O2 together accounted for 88.9% (n=27) of CRKP isolates. Genes encoding yersiniabactin (ybt) and aerobactin (iuc) were identified in 88.9% (24/27) and 29.6% (8/27) of isolates. The predominant plasmid replicons present were ColKP3 (55.5%), IncFII(K) (51.8%) and IncFIB(pQil) (44.4%).

conclusionsThis study emphasises the need for continued genomic surveillance of MDR bacteria that could be instrumental in developing treatment guidelines based on integrating phenotypic and molecular methods.

Indexed as

Carbapenem-Resistant EnterobacteriaceaeKlebsiella InfectionsAnti-Bacterial Agentsbeta-LactamasesCarbapenemsCross-Sectional StudiesHospitals, TeachingHumansKlebsiella pneumoniaeMicrobial Sensitivity TestsTertiary Health CareAnti-Bacterial Agentsbeta-LactamasesCarbapenemscarbapenem resistanceKlebsiella pneumoniaemolecular characterisation

Identifiers

PMID35029688
PMCPMC9259183

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