ArticleJournal of experimental botany2022
A naturally evolved mutation (Ser59Gly) in glutamine synthetase confers glufosinate resistance in plants.
Article in Journal of experimental botany, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 43 citations in OpenAlex.
- Simultaneous detection of glyphosate and glufosinate target-site resistance in Eleusine indica via multiplex TaqMan qPCR.Pest management science · 2026Article
- Analysis of glutamine synthetase target-site mutations and their role in endowing glufosinate-ammonium resistance.Pest management science · 2026Article
- Widespread non-target-site resistance in Setaria viridis to four classes of herbicide.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Shared Plant-human Biology: Herbicide Effects and New Biomarkers Perspectives.Current environmental health reports · 2026Review
- CRISPR/Cas9-Mediated Base Editing of SiGS1 Confers Glufosinate Resistance in Foxtail Millet (Setaria italica).Plant biotechnology journal · 2026Article
- Chromosome-level genome assembly of triploid Cyperus rotundus uncovers allelic expression patterns and herbicide resistance mechanisms.Plant communications · 2026Article
- Genome Editing Enhanced Abiotic Stress ToleranceIn Cereal Crops.Functional & integrative genomics · 2025Review
- Cationic amino acid transporters (CAT) enhance accumulation and susceptibility to the systemic herbicide L-phosphinothricin.Nature communications · 2025Article
- A rearranged Amaranthus palmeri extrachromosomal circular DNA confers resistance to glyphosate and glufosinate.The Plant cell · 2025Article
- Breeding herbicide-resistant rice using CRISPR-Cas gene editing and other technologies.Plant communications · 2025Review
- Mutation of GLR2 confers enhanced glufosinate resistance and salt tolerance in rice.Plant physiology · 2024Article
- Synthetic directed evolution for targeted engineering of plant traits.Frontiers in plant science · 2024Review
- Molecular Targets of Herbicides and Fungicides─Are There Useful Overlaps for Fungicide Discovery?Journal of agricultural and food chemistry · 2023Review
- Hormonal Status of Transgenic Birch with a Pine Glutamine Synthetase Gene during Rooting In Vitro and Budburst Outdoors.Biomolecules · 2023Article
- Precision editing of GLR1 confers glufosinate resistance without yield penalty in rice.Plant biotechnology journal · 2023Article
- Effects of Biological Nitrogen Metabolism on Glufosinate-Susceptible and -Resistant Goosegrass (International journal of molecular sciences · 2023Article
- Genomic analyses provide insights into the polyploidization-driven herbicide adaptation in Leptochloa weeds.Plant biotechnology journal · 2023Article
- Genetic Analysis and Fine Mapping ofInternational journal of molecular sciences · 2022Article
- Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
Glufosinate is an important and widely used non-selective herbicide active on a wide range of plant species. Evolution of resistance to glufosinate in weedy plant species (including the global weed Eleusine indica) is underway. Here, we established the molecular basis of target site glufosinate resistance in Eleusine indica. Full-length E. indica glutamine synthetase (GS) iso-genes (EiGS1-1, 1-2, 1-3, and EiGS2) were cloned, and expression of EiGS1-1 and EiGS1-2 was higher than that of EiGS2. A novel point mutation resulting in a Ser59Gly substitution in EiGS1-1 was identified in glufosinate-resistant plants. Rice calli and seedlings transformed with the mutant EiGS1-1 gene were resistant to glufosinate. Purified mutant EiGS1-1 expressed in yeast was more tolerant to glufosinate than the wild-type variant. These transgenic results correlate with a more glufosinate-resistant GS in the crude tissue extract of resistant versus susceptible E. indica plants. Structural modelling of the mutant EiGS1-1 revealed that Ser59 is not directly involved in glufosinate binding but is in contact with some important binding residues (e.g. Glu297) and especially with Asp56 that forms an intratoroidal contact interface. Importantly, the same Ser59Gly mutation was also found in geographically isolated glufosinate-resistant populations from Malaysia and China, suggesting parallel evolution of this resistance mutation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.