Evidence map›Paper›PMID 35028691›Full record

ArticleAnalytical and bioanalytical chemistry2022

A biomimetic enzyme-linked immunosorbent assay (BELISA) for the analysis of gonadorelin by using molecularly imprinted polymer-coated microplates.

Francesca Torrini, Laura Caponi, Andrea Bertolini, Pasquale Palladino, Francesca Cipolli, Alessandro Saba, Aldo Paolicchi, Simona Scarano, Maria Minunni

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 18 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Francesca TorriniDepartment of Chemistry 'Ugo Schiff', University of Florence, Sesto Fiorentino (FI), Italy.
Laura CaponiLaboratory of Clinical Pathology, University Hospital of Pisa, Pisa, Italy.
Andrea BertoliniDepartment of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, Pisa, Italy.
Pasquale PalladinoDepartment of Chemistry 'Ugo Schiff', University of Florence, Sesto Fiorentino (FI), Italy.
Francesca CipolliLaboratory of Clinical Pathology, University Hospital of Pisa, Pisa, Italy.
Alessandro SabaDepartment of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, Pisa, Italy.
Aldo PaolicchiLaboratory of Clinical Pathology, University Hospital of Pisa, Pisa, Italy.
Simona ScaranoDepartment of Chemistry 'Ugo Schiff', University of Florence, Sesto Fiorentino (FI), Italy. simona.scarano@unifi.it.
Maria MinunniDepartment of Chemistry 'Ugo Schiff', University of Florence, Sesto Fiorentino (FI), Italy. maria.minunni@unifi.it.
Azienda Ospedaliera Universitaria Pisana · ITUniversity of Florence · ITUniversity of Pisa · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

An original biomimetic enzyme-linked immunoassay (BELISA) to target the small peptide hormone gonadorelin is presented. This peptide has been recently listed among the substances banned in sports by the World Antidoping Agency (WADA) since its misuse by male athletes triggers testosterone increase. Hence, in response to this emerging issue in anti-doping controls, we proposed BELISA which involves the growth of a polynorepinephrine (PNE)-based molecularly imprinted polymer (MIP) directly on microwells. PNE, a polydopamine (PDA) analog, has recently displayed impressive performances when it was exploited for MIP preparation, giving even better results than PDA. Gonadorelin quantification was accomplished via a colorimetric indirect competitive bioassay involving the competition between biotinylated gonadorelin linked to the signal reporter and the unlabeled analyte. These compete for the same MIP binding sites resulting in an inverse correlation between gonadorelin concentration and the output color signal (λ = 450 nm). A detection limit of 277 pmol L

Indexed as

BiomimeticsMolecular ImprintingEnzyme-Linked Immunosorbent AssayGonadotropin-Releasing HormoneHumansMaleMolecularly Imprinted PolymersReproducibility of ResultsGonadotropin-Releasing HormoneMolecularly Imprinted PolymersAntibody mimeticsEnzyme-linked immunosorbent assayGonadotropin-releasing hormoneMolecularly imprinted polymersPolydopaminePolynorepinephrine

Identifiers

PMID35028691
PMCPMC9242967
OpenAlexW4205635888

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.