ArticleJournal of dental sciences2022
Migration and phenotype switching of macrophages at early-phase of bone-formation by secretomes from bone marrow derived mesenchymal stem cells using rat calvaria bone defect model.
Article in Journal of dental sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Monocyte chemoattractant protein-1 from a conditioned medium of bone marrow-derived mesenchymal stem cells promotes bone regeneration by enhancing macrophage phenotype polarization.Maxillofacial plastic and reconstructive surgery · 2026Article
- Single-cell RNA sequencing identifies M2-like macrophage polarization associated with mesenchymal stem cell treatment in a murine sepsis model.Biomolecules & biomedicine · 2026Article
- The utility of 3D nanostructured polyglycolic acid fibers for novel bone regenerative medicine.Journal of dental sciences · 2026Article
- Cyclooxygenase-2 as a potential therapeutic target in the treatment of chemoresistant glioblastomas.Medical oncology (Northwood, London, England) · 2025Review
- T cell related osteoimmunology in fracture healing: Potential targets for augmenting bone regeneration.Journal of orthopaedic translation · 2025Review
- Categories, applications, and potential of stem cells in bone regeneration: an overview.Frontiers in medicine · 2025Review
- Macrophages in osteoporotic fractures: from immunometabolic mechanisms to precision therapeutic approaches.Frontiers in endocrinology · 2025Review
- Review
- Mesenchymal stem cells suppress inflammation by downregulating interleukin-6 expression in intestinal perforation animal model.Annals of medicine and surgery (2012) · 2024Article
- Bone regeneration by a bone substitute biomaterial containing hydroxyapatite, chitosan, xanthan and graphene oxide supplemented with conditioned medium from mesenchymal stem cells.Acta odontologica latinoamericana : AOL · 2024Article
- Metabolites of Kimchi Lactic Acid Bacteria, Indole-3-Lactic Acid, Phenyllactic Acid, and Leucic Acid, Inhibit Obesity-Related Inflammation in Human Mesenchymal Stem Cells.Journal of microbiology and biotechnology · 2024Article
- Revolutionizing bone defect healing: the power of mesenchymal stem cells as seeds.Frontiers in bioengineering and biotechnology · 2024Review
- Assessment of rhBMP-2-loaded bovine hydroxyapatite granules in the guided bone regeneration of critical bone defect in rat mandible bone.Journal of dental sciences · 2024Article
- Distinct differences in immunological properties of equine orthobiologics revealed by functional and transcriptomic analysis using an activated macrophage readout system.Frontiers in veterinary science · 2023Article
- The Secretome of Preconditioned Mesenchymal Stem Cells Drives Polarization and Reprogramming of M2a Macrophages toward an IL-10-Producing Phenotype.International journal of molecular sciences · 2022Article
- Amnion and chorion matrix maintain hMSC osteogenic response and enhance immunomodulatory and angiogenic potential in a mineralized collagen scaffold.Frontiers in bioengineering and biotechnology · 2022Article
- Extracellular Vesicles From TNFα Preconditioned MSCs: Effects on Immunomodulation and Bone Regeneration.Frontiers in immunology · 2022Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background/purposeConditioned media of cultured mesenchymal stem cells (MSCs) contain numerous kinds of secretomes such as cytokines and chemokines. We previously reported that conditioned media of bone marrow-derived MSCs (MSC-CM) promote bone formation. Recently, macrophage phenotype switching from the pro-inflammatory M1 type to the anti-inflammatory M2 type has been reported to be an important phenomenon during tissue regeneration. Some studies reported that this phenotype switching is regulated by secretomes. In this study, macrophage phenotype during bone formation by MSC-CM was investigated. MATERIALS AND
methodsHuman MSCs (hMSCs) were cultured in serum-free medium and the collected medium was defined as MSC-CM. Macrophage-related gene expressions in hMSCs cultured with MSC-CM were evaluated by quantitative real-time polymerase chain reaction. MSC-CM was implanted and the evaluations by micro-CT and immunohistochemistry were performed using a rat the calvaria bone defect model.
resultsTwo and four weeks after implantation, the MSC-CM group demonstrated enhanced bone regeneration. Gene expressions of
conclusionMSC-CM enhances macrophage migration and induces M1 to M2 type macrophage switching at an early stage of osteogenesis. Such phenotype switching provides a favorable environment for angiogenesis, cellular migration, and osteogenesis and contributes to MSC-CM-induced early bone formation.
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