ArticleJournal of biomolecular techniques : JBT2021
RT qLAMP-Direct Detection of SARS-CoV-2 in Raw Sewage.
Article in Journal of biomolecular techniques : JBT, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Remotely Controlled Continuous Surveillance of Viral RNA in Wastewater Using a LoRa Network.IEEE internet of things journal · 2026Article
- Comparative evaluation of heating instruments for detectingJournal of water and health · 2025Article
- Article
- A Narrative Review of High Throughput Wastewater Sample Processing for Infectious Disease Surveillance: Challenges, Progress, and Future Opportunities.International journal of environmental research and public health · 2024Review
- [Diagnosis by isothermal amplification of nucleic acids. Opportunity for community pharmacies].Farmaceuticos comunitarios · 2024Review
- Wastewater-based surveillance as a tool for public health action: SARS-CoV-2 and beyond.Clinical microbiology reviews · 2024Review
- Integration of RT-LAMP and Microfluidic Technology for Detection of SARS-CoV-2 in Wastewater as an Advanced Point-of-Care Platform.Food and environmental virology · 2022Article
- The Future of Point-of-Care Nucleic Acid Amplification Diagnostics after COVID-19: Time to Walk the Walk.International journal of molecular sciences · 2022Review
- An isothermal lab-on-phone test for easy molecular diagnosis of SARS-CoV-2 near patients and in less than 1 hour.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2022Article
- A quantitative RT-qLAMP for the detection of SARS-CoV-2 and human gene in clinical application.Microbial biotechnology · 2022Article
- Improved methods for the detection and quantification of SARS-CoV-2 RNA in wastewater.Scientific reports · 2022Article
- Molecular Diagnostic Tools Applied for Assessing Microbial Water Quality.International journal of environmental research and public health · 2022Review
- Nanobiotechnology enabled approaches for wastewater based epidemiology.Trends in analytical chemistry : TRAC · 2021Review
- Wastewater-Based Epidemiology as an Early Warning System for the Spreading of SARS-CoV-2 and Its Mutations in the Population.International journal of environmental research and public health · 2021Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A highly efficient, selective, and sensitive method for analysis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in raw sewage was developed and tested to illustrate basic characteristics of the procedure. The method uses reverse transcriptase (RT) loop mediated isothermal amplification (LAMP) in a quantitative application, RT qLAMP. The applicability of this procedure to detection of SARS-CoV-2 in clinical samples has been documented in many reports since early 2020. Basic LAMP characteristics depending on the multiple primer design that produce highly selective and sensitive target amplification virtually free of interferences in complex sample media make it ideal for application to target recognition in raw sewage. Three previously described primer sets targeting ORF1a, E- and N-gene regions were selected and tested to define method performance characteristics and performance for SARS-CoV-2 detection in raw sewage samples from a municipal sewage system serving > 600 000, between July and October, 2020. The virus was detected in all samples from each of three independent interceptors near their treatment terminus. Virus quantities varied significantly between samples and between primer targets within samples. Sewage sampling dates corresponded to relatively low COVID-19 incidence rates reported by the local service area health department. The limited number of samples and aggregating downstream sampling locations did not permit resolving concentration differences. The most significant finding was the ability of the RT qLAMP method to detect SARS-CoV-2 in the raw sewage samples directly without preprocessing to isolate or concentrate the virus or to extract and concentrate viral RNA.
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